Cell Cycle Control: Methods and Protocols

ISBN-13: 9781071635568 / Angielski

Anna Castro; Benjamin LaCroix

Spis treści

1. Single Molecule Approaches to Study DNA Condensation

            Stefan Golfier, Thomas Quail, and Jan Brugués

 

2. Studying Translesion DNA Synthesis Using Xenopus In Vitro Systems

            Antoine Aze, James R.A. Hutchins, and Domenico Maiorano

 

3. Cell-Cycle Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modified Cell Lines

            Dorothee Kommer, Konstantinos Stamatiou, and Paola Vagnarelli

 

4. Dissecting the Multiple Functions of the Polo-Like Kinase 1 (PLK-1) in the C. elegans Zygote

            Griselda Velez-Aguilera, Batool Ossareh-Nazari, and Lionel Pintard

 

5. Artificial Modulation and Rewiring of Cell Cycle Progression Using Synthetic Circuits in Fission Yeast

            Akanksha Jain, Pei-Yun Jenny Wu, and Damien Coudreuse

 

6. Measuring Molecular Diffusion in Self‐Organizing Xenopus Extracts by Fluorescence Correlation Spectroscopy

            William Y.C. Huang, James E. Ferrell, Jr., and Xianrui Cheng

 

7. Mechanical Characterization of Murine Oocytes by Atomic Force Microscopy

            Rose Bulteau, Lucie Barbier, Guillaume Lamour, Tristan Piolot, Elsa Labrune, Clément Campillo, and Marie-Emilie Terret

 

8. Manipulation of Embryonic Cleavage Geometry Using Magnetic Tweezers

            Jing Xie, Daniel L. Levy, Nicolas Minc, and Jérémy Sallé

 

9. Crosstalk Between Metabolism and the Cell Division Cycle

            Diana Vara-Ciruelos and Marcos Malumbres

 

10. Give and Take: The Reciprocal Control of Metabolism and Cell Cycle

            Romain Riscal, Blanche Riquier-Morcant, Gilles Gadea, and Laetitia Linares

 

11. Preparation of Xenopus borealis and Xenopus tropicalis Egg Extracts for Comparative Cell Biology and Evolutionary Studies

            Maiko Kitaoka, Gabriel Guilloux, Rebecca Heald, and Romain Gibeaux

 

12. Measuring Mitotic Spindle and Microtubule Dynamics in Marine Embryos and Non-Model Organisms

            Janet Chenevert, Morgane L.V. Robert, Jérémy Sallé, Sébastien Cacchia, Thierry Lorca, Anna Castro, Alex McDougall, Nicolas Minc, Stefania Castagnetti, Julien Dumont, and Benjamin Lacroix

 

13. Whole Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis

            Safia El Jailani, Katja Wassmann, and Sandra Touati

 

14. Imaging and Analysis of Drosophila Neural Stem Cell Asymmetric Division

            Anne-Marie Berisha, Gregory Eot-Houllier, and Régis Giet

 

15. Cell Cycle Mapping Using Multiplexed Immunofluorescence

            Katarzyna M. Kedziora and Wayne Stallaert

 

16. Investigating Heterogeneous Cell-Cycle Progression Using Single-Cell Imaging Approaches

            Hee Won Yang

 

17. MAARS Software for Automatic and Quantitative Analysis of Mitotic Progression

            Tong Li, Yannick Gachet, and Sylvie Tournier

Blurb

This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and cellular types used to visualize cellular architecture during cell division, as well as innovative single cell microscopy techniques to highlight the heterogeneity of the cell population with respect to cell cycle progression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell Cycle Control: Methods and Protocols serves as an ideal guide for researchers attempting to elucidate this vital area of cell biology.