Cell Cycle Control: Mechanisms and Protocols

ISBN-13: 9781617373855 / Angielski / Miękka / 2010 / 404 str.

Tim Humphrey; Gavin Brooks

Opis

The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that all cells come from cells. In recent years, considerable effort has been applied to the identification of the basic molecules and mechanisms that regulate the cell cycle in a number of different organisms. Such studies have led to the elucidation of the central paradigms that underpin eukaryotic cell cycle control, for which Lee Hartwell, Tim Hunt, and Paul Nurse were jointly awarded the Nobel Prize for Medicine and Physiology in 2001 in recognition of their seminal contributions to this field. The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development, and cell death. This new phase of integrated cell cycle research provides further challenges and opportunities to the biological and medical worlds in applying these basic concepts to understanding the etiology of cancer and other proliferative diseases."

Spis treści

Part I. Overviews of Cell Cycle Control in Different Organisms Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce The Plant Cell Cycle: An Overview John H. Doonan The C. elegans Cell Cycle: Overview of Molecules and Mechanisms Sander van den Heuvel Developmental Control of Growth and Cell Cycle Progression in Drosophila Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio The Xenopus Cell Cycle: An Overview Anna Philpott and P. Renee Yew The Mammalian Cell Cycle: An Overvew Jane V. Harper and Gavin Brooks Part II. Cell Cycle Study Methods and Techniques Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle Jane V. Harper Mapping Origins of DNA Replication in Eukaryotes Susan A. Gerbi In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast Stephen E. Kearsey, Lydia Brimage, Mandana Namdar, Emma Ralph, and Xiaowen Yang Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs Peter D. Adams The Cell Cycle and Virus Infection Stevan R. Emmett, Brian Dove, Laura Mahoney, Torsten Wurm, and Julian A. Hiscox Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies Julie Welburn and Jane Endicott Part III. Detection and Measurement of Cell Cycle Molecules E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression Ludger Hauck and Rüdiger von Harsdorf Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors Katrina A. Bicknell Measurement of Geminin Activity in Xenopus Egg Extracts Thomas J. McGarry CDK-ActivatingKinases: Detection and Activity Measurements Stéphane Larochelle and Robert P. Fisher Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements Gavin Brooks Measurement of Wee Kinase Activity Paul R. Mueller and Walter F. Leise III CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements Sorab N. Dalal and Melanie Volkening Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1 Carmela Palermo and Nancy C. Walworth Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown Marcel A. T. M. van Vugt and René H. Medema The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs Mark L. McCleland and P. Todd Stukenberg Index

Blurb

Our understanding of the cell cycle and its regulation has significantly increased with recent discoveries of links to DNA repair, growth, cellular metabolism, development, cell death, proliferative disease, cardiovascular diseases, and cancer. In Cell Cycle Control: Mechanisms and Protocols, internationally recognized researchers not only provide overviews of cell cycle regulatory mechanisms, but also set forth their best techniques for studying the eukaryotic cell cycle and its key regulatory molecules in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators-and those more recently defined-in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Cell Cycle Control: Mechanisms and Protocols offers all cell researchers an indispensable collection of critical reviews and techniques essential to investigate how normal cells divide and how this is altered in disease.