Cell Culture Techniques in Heart and Vessel Research

ISBN-13: 9783642752643 / Angielski / Miękka / 2011 / 362 str.

H. M. Piper

Opis

In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

Spis treści

Heart Muscle Cells.- Embryonic Chick Heart Muscle Cells.- Neonatal Rat Heart Muscle Cells.- Adult Ventricular Rat Heart Muscle Cells.- Adult Atrial Cardiocytes in Culture.- Muscle Cell Cultures from Human Heart.- Attachment Substrates for Heart Muscle Cells.- Microcarrier Culture of Neonatal Heart Cells.- Endothelial Cells.- Macrovascular Endothelial Cells from Porcine Aorta.- Microvascular Endothelial Cells from the Lungs.- Microvascular Endothelial Cells from Brain.- Microvascular Endothelial Cells from Heart.- Macro- and Microvascular Endothelial Cells from Human Tissues.- Vascular Endothelial Cell-Synthesized Extracellular Matrices as Attachment Substrates In Vitro.- Endothelial Cells Grown on Filter Membranes.- Microcarrier Cultures of Endothelial Cells.- Growth Factors for Vascular Endothelial Cells.- Smooth Muscle Cells and Pericytes.- Smooth Muscle Cells from Rabbit Aorta.- Smooth Muscle Cells from Adult Human Aorta.- Coculture of Endothelial and Smooth Muscle Cells.- Culture and Study of Pericytes.- Attachment Substrates for Smooth Muscle Cells.- Methods of Culturing Vascular Smooth Muscle Cells on Microcarriers.- Growth Factors for Smooth Muscle Cells.- List of Suppliers.