Cell Culture Techniques

ISBN-13: 9781617790768 / Angielski / Twarda / 2011 / 497 str.

Michael Aschner; Cristina Sunol; Anna Bal-Price

Opis

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory. Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges.

Spis treści

1. Guidance on Good Cell Culture Practice (GCCP)

            Anna Bal-Price and Sandra Coecke

 

2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology

            M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman

 

3. Neural Stem Cells

            Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra Ceccatelli

 

4. Primary Cultures for Neurotoxicity Testing

            Carme Solà, Rosa Cristòfol, Cristina Suñol, and Coral Sanfeliu

 

5. Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening

            Paul Honegger and Marie-Gabrielle Zurich

 

6. Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans

            Michelle L. Tucci, Guy A. Caldwell, and Kim A. Caldwell

 

7. Modeling the Blood-Brain Barrier

            Marie-Pierre Dehouck, Elodie Vandenhaute, Lucie Dehouck, Emmanuel Sevin, Anne-Marie Lenfant, Yannick Delplace, Dorothée Hallier-Vanuxeem, Maxime Culot, and Roméo Cecchelli

 

8. In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research

            Nathalie Strazielle and Jean-François Ghersi-Egea

 

9. Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies

            Marcelo Farina, Jordi Berenguer, Sebastián Pons, João Batista Teixeira da Rocha, and Michael Aschner

 

10. P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions

            Joseph Bressler, Cliona O’Driscoll, Cathleen Marshall, and Walter Kaufmann

 

11. Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems?

            Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti

 

12. Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy

            Anna Forsby

 

13. The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity

            Alan J. Hargreaves, Magda Sachana, and John Flaskos

 

14. Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture

            Michael D. Aleo

 

15. Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death

            Michael Fricker and Aviva M. Tolkovsky

 

16. Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures

            Jonas J. Neher, Guy C. Brown, Agnieszka Kinsner-Ovaskainen, and Anna Bal-Price

 

17. Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation

            Dejan Milatovic and Michael Aschner

 

18. Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology

            Jeannette N. Stankowski, Simona G. Codreanu, Daniel C. Liebler, and BethAnn McLaughlin

 

19. Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity

            Juan Segura-Aguilar

 

20. ¹³C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells

            Ursula Sonnewald, Arne Schousboe, and Helle S. Waagepetersen

 

21. Culture Models for the Study of Amino Acid Transport and Metabolism

            Marta Sidoryk-Węgrzynowicz and Michael Aschner

 

22. Neurotransmitter Transporters and Anticonvulsant Drug Development

            Arne Schousboe, Karsten K. Madsen, and H. Steve White

 

23. Ion Channel Electrophysiology in Cultured Neurons

            Toshio Narahashi

 

24. Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips

            Dieter G. Weiss

 

25. GABA_A Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing

            Cristina Suñol and Daniel A. García