Cell-Based Microarrays: Methods and Protocols

ISBN-13: 9781493957842 / Angielski / Miękka / 2016 / 209 str.

Ella Palmer

Opis

As a high-throughput method of analyzing gene function, cell-based microarrays have proven of vital importance. Here, experts provide an up-to-date assessment of these microarrays and meticulous coverage of all their aspects, including emerging technology.

Spis treści

1. Cell-Based Microarrays: Overview Ella Palmer 2. Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate Meenal Soni and Fang Lai 3. Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays Ella Palmer and Tom C. Freeman 4. A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays Tanya M. Redmond and Michael D. Uhler 5. High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays Yuhui Hu and Michal Janitz 6. Cell Arrays for the Measurement of Organelle Dynamics in Living Cells Holger Erfle, Tautvydas Lisauskas, Christoph Claas, Jürgen Reymann, and Vytaute Starkuviene 7. High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays Wei Niu, G. Traver Hart, and Edward M. Marcotte 8. Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA) Hansjürgen Volkmer and Frank Weise 9. Reverse Transfected Cell Microarrays in Infectious Disease Research Andreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, and Michael Stürzl 10. Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies Baochuan Lin and James B. Delehanty 11. Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging Yoann Roupioz, Sarah Milgram, André Roget, and Thierry Livache 12. Immobilized Culture and Transfection Microarray of Nonadherent Cells Satoshi Yamaguchi, Erika Matsunuma, and Teruyuki Nagamune 13. Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays Andrew L. Hook, Nicolas H. Voelcker, and Helmut Thissen 14. Polymer Microarrays for Cellular High-Content Screening Salvatore Pernagallo and Juan J. Diaz-Mochon 15. High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation Koichi Kato and Hiroo Iwata 16. Microfluidic Image Cytometry Ken-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, and Robert Damoiseaux

Blurb

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.