Cartilage Tissue Engineering: Methods and Protocols

ISBN-13: 9781493948970 / Angielski / Miękka / 2016 / 290 str.

Pauline M. Doran

Spis treści

Part I: Overview

1. Cartilage Tissue Engineering: What Have We Learned in Practice?
Pauline M. Doran

Part II: Cell Isolation, Expansion and Differentiation

2. Human Foetal and Adult Chondrocytes
Kifah Shahin, Nastaran Mahmoudifar, and Pauline M. Doran

3. Mesenchymal Stem Cells Derived from Human Bone Marrow
Oliver F. W. Gardner, Mauro Alini, and Martin J. Stoddart

4. Mesenchymal Stem Cells Derived from Human Adipose Tissue
Nastaran Mahmoudifar and Pauline M. Doran

5. Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors
Hicham Drissi, Jason D. Gibson, Rosa Guzzo, and Ren-He Xu

6. Differentiation of Human Induced Pluripotent Stem Cells to Chondrocytes
Rosa M. Guzzo and Hicham Drissi

7. Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis
Feng Zhang and Dong-An Wang

Part III: Biomaterials and Scaffolds

8. Hydrogels with Tunable Properties
Peggy P.Y. Chan

9. Decellularized Extracellular Matrix Scaffols for Cartilage Regeneration
Shraddha Thakkar, Hugo Fernandes, and Lorenzo Moroni

10. Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft

Ting Ting Lau, Wenyan Leong, Yvonne Peck, Kai Su, and Dong-An Wang

11. Bioprinted Scaffolds for Cartilage Tissue Engineering
Hyun-Wook Kang, James J. Yoo, and Anthony Atala

12. Scaffolds for Controlled Release of Cartilage Growth Factors
Marie Morille, Marie-Claire Venier-Julienne, and Claudia N. Montero-Menei

13. Nanostructured Capsules for Cartilage Tissue Engineering
Clara R. Correia, Rui L. Reis, and João F. Mano

14. Stratified Scaffolds for Osteochondral Tissue Engineering
Patcharakamon Nooeaid, Gundula Schulze-Tanzil, and Aldo R. Boccaccini

Part IV: Bioreactors

15. Mechanobioreactors for Cartilage Tissue Engineering
Joanna F. Weber, Roman Perez, and Stephen D. Waldman

16. Shear and Compression Bioreactor for Cartilage Synthesis
Kifah Shahin and Pauline M. Doran

17. Microbioreactors for Cartilage Tissue Engineering
Yu-Han Chang and Min-Hsien Wu

Part V: In Vivo Application

18. Transplantation of Tissue-Engineered Cartilage in an Animal Model (Xenograft and

Autograft): Construct Validation
Hitoshi Nemoto, Deborah Watson, and Koichi Masuda

Part VI: Evaluation

19. Proteomic Analysis of Engineered Cartilage
Xinzhu Pu and Julia Thom Oxford

20. Mechanical Testing of Cartilage Constructs
Dinorath Olvera, Andrew Daly, and Daniel John Kelly

O autorze

Pauline M. Doran Professor Faculty of Life and Social Sciences Swinburne University of Technology PO Box 218 Hawthorn VIC 3122 Australia Ph: +61-3-9214-5716 Email: pdoran@swin.edu.au

Blurb

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ‘smart’ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.