Cartilage Tissue Engineering

ISBN-13: 9781071628386 / Angielski / Twarda / 2022 / 393 str.

Martin J. Stoddart; Elena Della Bella; Angela R. Armiento

Opis

This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair.

Spis treści

1. Cartilage Tissue Engineering: An Introduction

            Martin J. Stoddart, Elena Della Bella, and Angela R. Armiento

 

2. Chondrocyte Isolation and Expansion

            Paul Harrison, Timothy Hopkins, Charlotte Hulme, Helen McCarthy, and Karina Wright

 

3. Isolation of Chondrons from Hyaline Cartilage

            Jasmijn V. Korpershoek, Margot Rikkers, and Lucienne A. Vonk

 

4. Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation

            Ilyas M. Khan, Joshua McKenna, and Yadan Zhang

 

5. Physioxic Culture of Chondrogenic Cells

            Girish Pattappa, Brandon D. Markway, Denitsa Docheva, and Brian Johnstone

 

6. Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

            Angela R. Armiento, Yann D. Ladner, Elena Della Bella, and Martin J. Stoddart

 

7. Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation

            Justyna M. Buchert, Benedict Lotz, Solvig Diederichs, and Wiltrud Richter

 

8. Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells

            Amanda R. Dicks, Nancy Steward, Farshid Guilak, and Chia-Lung Wu

 

9. The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans

            Yann D. Ladner, Mauro Alini, and Angela R. Armiento

 

10. Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy

            Alexander Otahal, Andrea De Luna, Ali Mobasheri, and Stefan Nehrer

 

11. Metabolomic Profiling to Understand Chondrocyte Metabolism

            Priyanka P. Brahmachary, Hope D. Welhaven, and Ronald K. June

 

12. Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes

            Huan Meng, Clare L. Thompson, Clarissa Coveney, Angus K. Wann, and Martin M. Knight

 

13. Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads

            Teresa Z. Brose, Yann D. Ladner, E. Johanna Kubosch, Martin J. Stoddart, and Angela R. Armiento

 

14. Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis

            Jillian L. McCool, Nicholas R. Hum, Aimy Sebastian, and Gabriela G. Loots

 

15. Modulation of MicroRNA Expression during In Vitro Chondrogenesis

            Austin Bell-Hensley, Hongjun Zheng, and Audrey McAlinden

 

16. Purification and Isolation of Proteins from Hyaline Cartilage

            Makenna J. Hardy, Xinzhu Pu, and Julia Thom Oxford

 

17. Histological Preparation and Evaluation of Cartilage Specimens

            Salim E. Darwiche, Milena Tegelkamp, Katja Nuss, and Brigitte von Rechenberg

 

18. Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology

            Bodo Kurz, Thomas Lange, Marita Voelker, and Bernd Rolauffs

 

19. Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization

            Seyed Ali Elahi, Rocío Castro-Viñuelas, Anke Govaerts, Rik Lories, Nele Famaey, and Ilse Jonkers

 

20. Viral Gene Delivery in Chondrocytes

            Christopher V. Nagelli, Christopher H. Evans, and Rodolfo E. De La Vega

 

21. Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy

            Cornelia Schneider and Sylvia Nürnberger

 

22. Self-Assembly Culture Model for Engineering Musculoskeletal Tissues

            Nicholas N. DePhillipo, Jerahme Martinez, and George R. Dodge

 

23. Osteochondral Explants Isolation and Culture under a Compression and Shear Bioreactor

            M. Letizia Vainieri and Sibylle Grad

 

24. A Mouse Model of Acute Cartilage Injury and Repair

            Anne-Sophie Thorup, Francesco Dell'Accio, and Suzanne E. Eldridge

 

25. Small-Animal Compression Models of Osteoarthritis

            Blaine A. Christiansen, Deva D. Chan, Marjolein C.H. van der Meulen, and Tristan Maerz

 

26. Modelling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM)

            Jadwiga Miotla-Zarebska, Ida Parisi, Vicky Batchelor, Tonia L. Vincent, and Paul K. Potter

 

27. Collagenase-Induced Osteoarthritis in Mice

            Peter M. van der Kraan

 

28. Platelet-Rich Plasma for Intra-Articular Injections: Preclinical and Clinical Evidence

            Angelo Boffa and Giuseppe Filardo