Cardiovascular Proteomics: Methods and Protocols

ISBN-13: 9781617376276 / Angielski / Miękka / 2010 / 432 str.

Fernando Vivanco

Opis

Over the past few years, the power and potential of proteomics has become widely recognized. The use of proteomics for the study of complex diseases is increasing and is particularly applicable to cardiovascular disease, the leading cause of death in developed countries. The ability to investigate the complete proteome provides a critical tool toward elucidating the complex and multif- torial basis of cardiovascular biology, especially disease processes. Proteomics involves the integration of a number of technologies with the aim of analyzing all the proteins expressed by a biological system in response to various stimuli under different pathophysiological conditions. The proteomic approach offers the ability to evaluate simultaneously the changes in protein expression and cell signaling pathways in response to such conditions as atherosclerosis, c- diac hypertrophy, stroke, or heart failure. Cardiovascular Proteomics: Methods and Protocols covers many of the above aspects of the proteomic approach in the cardiovascular field. This v- ume takes the reader through the complete process of proteomic analysis, from the obtention of specific heart proteins (troponin I) to the new techniques of identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, or the application of phage display techniques to decipher the molecular diversity of blood vessels.

Recenzje

"...a fine collection of methods and protocols currently employed in cardiovascular proteomics that will be of great use to all who are invovled in cardiovascular research." -Proteomics Clin. Appl.

"...a thorough set of protocols for the cardiac biology community.  Most laboratories will have some of these in place, but can profit from the broad coverage of this book." -Doody's Book Review, Weighted Numerical Score: 76 - 3 stars

Spis treści

Part I. Analysis of Heart Proteome Two-dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics Michael J. Dunn Analyzing The Cardiac Muscle Proteome By Liquid Chromatography-Mass Spectrometry (Lc-ms) Based Expression Proteomics Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. Maclennan, and Andrew Emili 2-D Differential Gel Electrophoresis (DIGE) of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, and Masaharu Kanoaka Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach Alberto Lázaro, Julio Gállego-delgado, Julio Osende, Jesús Egido, and Fernando Vivanco A Solubility Optimization Protocol for 2-D Gel Electrophoresis of Cardiac Tissue Brian A. Stanley and Jennifer E. Van Eyk A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium Brian A. Stanley and Jennifer E. Van Eyk Part II. Heart Subproteomes Subcellular Fractionation Mariana E. Guimarães De Araújo and Alfons Huber Subfractionation of Heart Tissue: The 'In Sequence' Myofilament Protein Extraction Of Myocardial Tissue Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and Seldi-tofT Diane E. Bovenkamp, Brian A. Stanley and Jennifer E.van Eyk Proteomic Analysis Of The Subunit Composition of Complex I (Nadh:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria Ian M. Fearnley, Joe Carrol and John E. Walker Identification of Targets of Phosphorylation in Heart Mitochondria Roberta A. Gottliebie Part III. Proteomics of Human Atheroma Plaques Characterization of The Human Atheroma Plaque Secretome by Proteomic Analysis Mari-CarmenDurán, Jose L. Martin-Ventura, Sebastián Mas, Maria G. Barderas, Verónica M. Dardé, Ole N. Jensen, Jesús Egido, and Fernando Vivanco Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martín-Ventura, Jesús Egido, Fernando Vivanco, and Ole N. Jensen Western Array Analysis of Human Atherosclerotic Plaque Wim Martinetar Part IV. The Proteome of Cells of The Cardiovascular System The Proteome of Endothelial Cells Jesús Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jesús Egido, and Fernando Vivanco Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells Richard R. Sprenger and Anton J. G. Horrevoets Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells Antonio Martínez-Ruiz and Santiago Lamasgo The Proteome and Secretome of Human Arterial Smooth Muscle Cell Annabelle Dupont and Florence Pinet Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle Anne A. Wooldridge and Timothy A. Haystead Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain Alexy V. Pshezhetsky Isolation and Culture of Adult Mouse Cardiac Myocytes Timothy D. O´Connell, Manoj C. Rodrigo, and Paul C. Simpson Proteomic Analysis Of Foam Cells Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin Isolation of The Platelet Releasate Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire Chapter 25 Verónica M. Dardé, Mari-Carmen Durán, Jesús Egido, and Fernando Vivanco Part V.