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Cardiovascular Development: Methods and Protocols
ISBN-13: 9781493958535 / Angielski / Miękka / 2016 / 341 str.
Cardiovascular Development: Methods and Protocols
ISBN-13: 9781493958535 / Angielski / Miękka / 2016 / 341 str.
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Including the step-by-step detail essential for reproducible results, this volume deals with the latest techniques in the field of cardiovascular development and includes key notes and implementation advice from experts in this high-profile medical field.
From the reviews:
"This is an up-to-date review of current research techniques used in the field of cardiovascular development. ... The book successfully instructs researchers on proper experimental techniques, giving step-by-step instructions, required for research in this field. ... this book is intended for use by both novice and experienced basic science researchers. ... This book is an excellent reference for any researcher in this area." (Patrick T. Campbell, Doody's Book Reviews, April, 2012)Preface
Contributors
Part I: Model organisms
1. Use of Whole Embryo Culture for Studying Heart Development
Calvin T. Hang, and Ching-Pin Chang
2. Quantifying Cardiac Functions in Embryonic and Adult Zebrafish
Tiffany Hoage, Yonghe Ding, and Xiaolei Xu
3. Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting
Ching-Pin Chang
4. Morpholino Injection in Xenopus
Panna Tandon, Chris Showell, Kathleen Christine, and Frank L Conlon
5. Chicken Chorioallantoic Membrane Angiogenesis Model
Domenico Ribatti
6. Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography
Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin
7. Whole-mount Confocal Microscopy for Vascular Branching Morphogenesis
Yoh-suke Mukouyama, Jennifer James, Joseph Nam, and Yutaka Uchida
8. Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining
Yang Liu, Marc Antoniyak, and Xu Peng
9. Miniaturized Assays of Angiogenesis in Vitro
May J. Reed and Robert B. Vernon
Part II: Cell and Molecular Biology Methods
10. Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay
Ching-Pin Chang, Yiqin Xiong, and Bin Zhou.
11. Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility
Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor
12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining
Yanping Zhang and L. Bruno Ruest
13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte
Jieli Li, Marc Antonyak, and Xu Peng
14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung
Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng
15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells
Bin Zhou and William T. Pu
16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
Jan Patterson, Rick Metz, and Emily Wilson
17. C-kit Expression Identifies Cardiac Precursor Cells
Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny
18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols
Dongfei Qi and Mingui Fu
19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels
Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja
20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups
Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,
Xu Peng, and David E. Dostal
Part III: New Techniques
21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways
Shenyuan Zhang and Hongying Zheng
22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells
Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy
23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy
Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal
24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis
Eric A. Bridenbaugh
25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research
Vincent VanBuren
26. High-speed Confocal Imaging of Zebrafish Heart Development
Michael P. Craig and Jay R. Hove
27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
David G. Taylor and Anupama Natarajan
Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor
12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining
Yanping Zhang and L. Bruno Ruest
13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte
Jieli Li, Marc Antonyak, and Xu Peng
14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung
Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng
15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells
Bin Zhou and William T. Pu
16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
Jan Patterson, Rick Metz, and Emily Wilson
17. C-kit Expression Identifies Cardiac Precursor Cells
Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny
18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols
Dongfei Qi and Mingui Fu
19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels
Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja
20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups
Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,
Xu Peng, and David E. Dostal
Part III: New Techniques
21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways
Shenyuan Zhang and Hongying Zheng
22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells
Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy
23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy
Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal
24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis
Eric A. Bridenbaugh
25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research
Vincent VanBuren
26. High-speed Confocal Imaging of Zebrafish Heart Development
Michael P. Craig and Jay R. Hove
27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
David G. Taylor and Anupama Natarajan
Bin Zhou and William T. Pu
16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
Jan Patterson, Rick Metz, and Emily Wilson
17. C-kit Expression Identifies Cardiac Precursor Cells
Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny
18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols
Dongfei Qi and Mingui Fu
19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels
Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja
20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups
Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,
Xu Peng, and David E. Dostal
Part III: New Techniques
21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways
Shenyuan Zhang and Hongying Zheng
22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells
Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy
23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy
Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal
24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis
Eric A. Bridenbaugh
25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research
Vincent VanBuren
26. High-speed Confocal Imaging of Zebrafish Heart Development
Michael P. Craig and Jay R. Hove
27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
David G. Taylor and Anupama Natarajan
Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy
23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy
Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal
24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis
Eric A. Bridenbaugh
25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research
Vincent VanBuren
26. High-speed Confocal Imaging of Zebrafish Heart Development
Michael P. Craig and Jay R. Hove
27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
David G. Taylor and Anupama Natarajan
Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal
24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis
Eric A. Bridenbaugh
25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research
Vincent VanBuren
26. High-speed Confocal Imaging of Zebrafish Heart Development
Michael P. Craig and Jay R. Hove
27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
David G. Taylor and Anupama Natarajan
Michael P. Craig and Jay R. Hove
27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays
David G. Taylor and Anupama Natarajan
David G. Taylor and Anupama Natarajan
Congenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and techniques for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.
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