Cardiac Tissue Engineering: Methods and Protocols

ISBN-13: 9781493910465 / Angielski / Twarda / 2014 / 266 str.

Milica Radisic; Lauren D. Black III

Opis

Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Spis treści

1.       Second Generation Codon Optimized Minicircle (Comic) For Non-Viral Reprogramming Of Human Adult Fibroblasts

Sebastian Diecke, Leszek Lisowski, Nicholas M. Mordwinkin, Nigel G. Kooreman, and Joseph C. Wu

 

2.       Scalable Cardiac Differentiation Of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates

Celine Bauwens and Mark Ungrin

 

3.       Preparation And Characterization Of Circulating Angiogenic Cells For Tissue Engineering Applications

Aleksandra Ostojic, Suzanne Crowe, Brian Mcneill, Marc Ruel, and Erik J. Suuronen

 

4.      Isolation And Expansion Of C-Kit Positive Cardiac Progenitor Cells By Magnetic Cell Sorting

Kristin M. French and Michael E. Davis

 

5.      Synthesis Of Aliphatic Polyester Hydrogel For Cardiac Tissue Engineering

Sanjiv Dhingra, Richard D. Weisel, and Ren-Ke Li

 

6.      Fabrication Of Pegylated Fibrinogen, A Versatile Injectable Hydrogel Biomaterial

Iris Mironi-Harpaz, Alexandra Berdichevski, and Dror Seliktar

 

7.       Native Cardiac Extracellular Matrix Hydrogels For Cultivation Of Human Stem Cell-Derived Cardiomyocytes

Donald O Freytes, John D O’Neill, Yi Duan-Arnold, Emily Wrona, and Gordana Vunjak-Novakovic

 

8.      Magnetically-Actuated Alginate Scaffold: A Novel Platform For Promoting Tissue Organization And Vascularisation

Yulia Sapir, Emil Ruvinov, Boris Polyak, and Smadar Cohen

9.      Shrink-Induced Biomimetic Wrinkled Substrates For Functional Cardiac Cell Alignment and Culture

Nicole Mendoza, Roger Tu, Aaron Chen, Eugene Lee, and Michelle Khine 

 

10.   Injectable ECM Scaffolds For Cardiac Repair

Todd Johnson, Rebecca Braden, and Karen Christman

 

11.   Generation Of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT)

Sebastian Schaaf, Alexandra Eder, Ingra Vollert, Andrea Stöhr, Arne Hansen, and Thomas Eschenhagen

 

12.  Cell Tri-Culture For Cardiac Vascularization

Ayelet Lesman, Lior Gepstein, and Shulamit Levenberg

 

13.  Cell Sheet Technology For Cardiac Tissue Engineering

Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Kenjiro Tadakuma, Masayuki Yamato, Makoto Kaneko, and Teruo Okano

 

14.  Design And Fabrication Of Biological Wires

Jason W. Miklas, Sara S. Nunes, Boyang Zhang, and  Milica Radisic

 

15.   Collagen-Based Engineered Heart Muscle

Malte Tiburcy, Tim Meyer, Poh Loong Soong, and Wolfram-Hubertus Zimmermann

 

16.   Creation Of A Bioreactor For The Application Of Variable Amplitude Mechanical Stimulation Of Fibrin-Gel Based Engineered Cardiac Tissue

Kathy Y. Morgan and Lauren D. Black III

 

17.   Preparation Of Acellular Myocardial Scaffolds With Well-Preserved Cardiomyocyte Lacunae, And Method For Applying Mechanical And Electrical Simulation To Tissue Construct

Bo Wang, Lakiesha N. Williams,Amy L. De Jongh Curry, and Jun Liao

 

18.  Patch-Clamp Technique In ESC-Derived Cardiomyocytes

Jie Liu and Peter H. Backx

 

19.  Optogenetic Control Of Cardiomyocytes

Christina M. Ambrosi and Emilia Entcheva

 

20.  Methods For Assessing The Electromechanical Integration Of Human Pluripotent Stem Cell-Derived Cardiomyocyte Grafts

Wei-Zhong Zhu, Dominic Filice, Nathan J. Palpant, and Michael A. Laflamme

 

21.   Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells In Micropatterned Cell Pairs

Hung Nguyen, Nima Badie, Luke Mcspadden, Dawn Pedrotty, and Nenad Bursac