2. Apical Resection and Cryoinjury in Neonatal Mouse Heart
Hua Shen, Ali Darehzereshki, Henry M. Sucov, and Ching-Ling Lien
3. Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice
Tilman Ziegler, Karl-Ludwig Laugwitz, and Christian Kupatt
4. Myocardial Infarction in Pigs
Andrea Bähr, Nadja Hornaschewitz, and Christian Kupatt
5. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish
Ines J. Marques, Andrés Sanz-Morejón, and Nadia Mercader
6. Cardiac Resection Injury in Zebrafish
Delicia Z. Sheng, Dawei Zheng, and Kazu Kikuchi
7. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish
Fei Sun, Adam R. Shoffner, and Kenneth D. Poss
8. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration
Yijen L. Wu
Part II: Ex Vivo and In Vivo Approaches
9. Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes
Marina Leone and Felix B. Engel
10. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes
Lu Han, Jocelyn Mich-Basso, and Bernhard Kühn
11. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells
Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, and Ravi Karra
12. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells
Jun Xu, Li Wang, Jiandong Liu, and Li Qian
13. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs
Abbigail Helfer and Nenad Bursac
14. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells
Ling Gao and Jianyi Zhang
15. Isolation[SL1] [DC2] and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts
Honghai Liu, Kevin Bersell, and Bernhard Kühn
16. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish
Sierra Duca and Jingli Cao
17. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters
Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, and Sean M. Wu
Part III: Visualizing and Manipulating Heart Regeneration
18. In Vivo Clonal Analysis of Cardiomyocytes
Ngoc B. Nguyen, G. Esteban Fernandez, Yichen Ding, Tzung Hsiai, and Reza Ardehali
19. High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry
Frank Gyngard, Louise Trakimas, and Matthew L. Steinhauser
20. AAV Gene Transfer to the Heart
Suya Wang, Yuxuan Guo, and William T. Pu
21. In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy
Nishat Sultana, Mohammad Tofael Kabir Sharkar, Yoav Hadas, Elena Chepurko, and Lior Zangi
22. Generation and Manipulation of Exosomes
Shiqi Hu, Li Qiao, and Ke Cheng
23. Epigenetic Assays in Purified Cardiomyocyte Nuclei
Matthew C. Hill and James F. Martin
24. Genetic Lineage Tracing of Non-Cardiomyocytes in Mice
Zhongming Chen and Jop H. van Berlo
25. Experimental Hypoxia as a Model for Cardiac Regeneration in Mice
Yuji Nakada and Hesham A. Sadek
[SL1]
[DC2]
This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them.
The chapter “Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.