Cardiac Cell and Gene Transfer

ISBN-13: 9781617372926 / Angielski / Miękka / 2010 / 253 str.

Joseph M. Metzger

Opis

Heart disease is the leading cause of death in developed countries. Recent experimental advances featuring cellular, molecular, and genetic tools and technologies offer the potential for new therapeutic strategies directed toward remediation of inherited and acquired heart diseases. Whether these recent basic science advances will ultimately translate to clinical efficacy for patients with heart disease is unknown and is important to ascertain. Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications is designed to provide the reader with up-to-date coverage of a myriad of specific methodo- gies and protocols for gene and cell transfer to the myocardium. Each chapter features a Notes section that provides useful how to problem-solving insights that are often left unstated in standard published protocols. Cardiac Cell and Gene Transfer: Principles, Protocols, and Appli- tions addresses principles and applications of cell and gene transfer to the heart, including protocols for vector production and purification. Detailed step-by-step methods and applications for first/second-generation adenoviral vectors, adeno-associated vectors, gutted adenoviral vectors, and lentiviral vectors are included. Additionally, detailed methods for cardiac cell grafting and transplantation are provided, and these chapters highlight the prospects of cell-based therapies for cardiac repair. The book also covers specific in vivo techniques for cardiac gene transfer, and specifies subsequent cellular and organ-level physiological assessment techniques and protocols. Accordingly, this book is designed for basic science and clinical researchers in the academic, pharmaceutical, and biotechnology sectors of the cardiovascular community."

Recenzje

"The book is surprisingly up-to-date...it is quite unique, as there is no similar compendium of detailed protocols. I found the book to be highly readable. It is a particularly useful reference work for cardiovascular scientists who are already using gene transfer approaches in their research. It is also a great starting place for cardiovascular investigators who are just beginning to use these methods in their labs." -Doody's Health Sciences Book Review Journal

Spis treści

Part I. Vectors for Cardiac Gene Transfer: Production, Purification, and Applications Adenoviral Vectors: Production and Purification Faris P. Albayya and Joseph M. Metzger Gutted Adenoviral Vectors for Gene Transfer to Muscle Jeannine M. Scott and Jeffrey S. Chamberlain Dual Vector Expansion of the Recombinant AAV Packaging Capacity Dongsheng Duan, Yongping Yue, and John F. Engelhardt Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes Tsuyoshi Sakoda, Noriyuki Kasahara, and Larry Kedes Part II. Cell Transfer to Myocardium: Protocols and Applications Cell Therapy in the Heart: Cell Production, Transplantation, and Applications Kevin S. Cahill, Catalin Toma, Mark F. Pittenger, Paul D. Kessler, and Barry J. Byrne Cardiac Cell Transplantation: Protocols and Applications Steven M. White and William C. Claycomb Cell Grafting for Cardiac Repair Hans Reinecke and Charles E. Murry Part III. Vectors for Cardiac Gene Transfer: In Vitro and In Vivo Assays and Approaches Suppressor tRNAs: Protocols and Applications for Cardiac Gene Transfer Massimo Buvoli, Ada Buvoli, and Leslie A. Leinwand Antisense Oligonucleotides: Design, Construction, and Applications to Cardiac Allograft Transfer Leora B. Balsam, Douglas N. Miniati, and Robert C. Robbins Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants Guanyi Lu and D. Keith Bishop Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes Jennifer C. Hirsch, Andrea R. Borton, and Joseph M. Metzger Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes Margaret V. Westfall Part IV. In Vivo Cardiac Gene Transfer: Protocols, Applications, and Physiological Assessment Embryonic and Neonatal Cardiac Gene Transfer In Vivo Geir Christensen, Peter J. Gruber, Yibin Wang, and Kenneth R. Chien Efficient Viral Gene Transfer to Rodent Hearts In Vivo Federica del Monte and Roger J. Hajjar Direct Gene Transfer to the Adult Rodent Myocardium In Vivo Michael L. Szatkowski, Margaret V. Westfall, and Joseph M. Metzger Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures Damien Logeart and Jean-Jacques Mercadier Coronary Perfusion Cocktails for In Vivo Gene Transfer Stephan E. Lehnart and J. Kevin Donahue Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of b-Adrenergic Receptor Signaling Components Hendrik T. Tevaearai and Walter J. Koch Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo Dimitrios Georgakopoulos and David A. Kass Index

Blurb

Cellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many novel techniques and strategies for the cellular and genetic modification of heart function. These highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro.
Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.