Capillary Gel Electrophoresis

ISBN-13: 9780444522344 / Angielski / Twarda / 2021 / 390 str.

Andras Guttman

Opis

"Capillary Gel Electrophoresis and Related Microseparation Techniques" covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity based methodologies, micropreparative aspects, as well as related microseparation methods. It gives readers a better understanding of how to utilize this technology, and determine which method will provide the best technical solutions to their particular problems.

This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology, and biotechnology courses.
Covers all theoretical and practical aspects of capillary gel electrophoresisExcellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity based methodologies, micropreparative aspects, as well as related microseparation methodsTeaches readers how to use the technology, and how to select methods ideal for fundamental problemsCan serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology, and biotechnology courses

Spis treści

1. Introduction
2. Separation Matrix and Column Technology
3. Instrumentation
4. Applications
5. Micropreparative Capillary Gel Electrophoresis
6. Related Microseparation Techniques
7. Appendix. Addresses of Some Suppliers of Instrumentation, Chemicals and Reagents

O autorze

András Guttman, MTA-Lendulet Professor of Translational Glycomics at the Research Institute for Biomolecular and Chemical Engineering at University of Pannonia (Veszprem, Hungary), also heading the Horváth Csaba Memorial Laboratory of Bioseparation Sciences. His work is focused on capillary gel electrophoresis for glycomics and glycoproteomics analysis of samples of biomedical and biopharmaceutical interests. Dr Guttman has over 350 scientific publications, wrote 35 book chapters, edited 4 textbooks and holds 25 patents. He is an external member of the Hungarian Academy of Sciences, Editor in Chief for Current Molecular Medicine, serves as editorial board member for a dozen scientific journals and on the board of several international scientific organizations. He has been recognized by numerous awards including the Analytical Chemistry Award of the Hungarian Chemical Society, named as Fulbright Scholar, received the CASSS CE Pharm Award, the Arany Janos medal of the Hungarian Academy of Sciences, the Pro Scientia award of the University of Pannonia and the Dennis Gabor Award of the Novofer Foundation. Dr Guttman is also the recipient of the Dal Nogare Award of the Delaware Valley Chromatography Forum and the Grand prize of the Swedish Chamber of Commerce. László Hajba holds a PhD in analytical and environmental chemistry from University of Pannonia, awarded in 2008, his research topic was the vibrational spectroscopic, theoretical and structural study of organometallic molecules. Later he carried out research in the field of biospectroscopy and chemometrics at the same university. He performed infrared spectroscopic investigation of human hair and skin, identification of cancer specific spectroscopic markers with chemometric methods. In 2010 he joined the biotechnology research group at Research Institute of Chemical and Process Engineering, University of Pannonia. In 2013 he started his work in the field of microfluidics and bioseparations at the Translational Glycomics Research Group. He and his coworkers developed a fully automated linear polyacrylamide capillary coating method for high efficiency capillary electrophoretic separation of cell surface proteins and the glycosimilarity index for the similarity measure of the glycan structures of therapeutic proteins. He received the Outstanding Scientist Award from the Hungarian Academy of Sciences in 2019. He has 47 peer reviewed scientific publication with more than 650 independent citations.