Capillary Electrophoresis: Methods and Protocols

ISBN-13: 9781588295392 / Angielski / Twarda / 2008 / 809 str.

Philippe Schmitt-Kopplin; Philippe Schmitt-Kopplin

Opis

Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. The volume is intended for beginners in the field and provides overviews of each technique and a starting point for the exploration of the defined literature on the different application topics. As a beginning text, each technique is explained in simple terms, assuming only minimal previous knowledge of the methods. A Notes section accompanies each chapter and addresses problems that may occur and how to overcome them. In Part I, Analyte-Oriented, contributors present methods and protocols based on sample type and size for inorganic and organic small ions, anionic, cationic, and neutral pollutants and pharmaceuticals, sugars, amino acids, nucleotides and DNA, and synthetic copolymers up to microorganisms. In Part II, Methods-Oriented, authors present the more methodological descriptions of particular and new CE techniques including CZE, MEKC/MECC, CEC, affinity CE, coating and online concentration techniques, single-cell analysis, and multidimensional techniques. Comprehensive and timely, Capillary Electrophoresis: Methods and Protocols is ideal for researchers looking for a beginning text on capillary electrophoresis methods.

Recenzje

From the reviews:

"The book Capillary Electrophoresis-Methods and Protocols gives a comprehensive overview of the recent state-of-the-art of CE methods for a wide range of components. ... One can consider the book as a capillary electrophoresis recipe book, and in this function it may serve very well for experienced CE users ... . the book is very detailed makes it a valuable addition to the library of any laboratory with CE-based research." (P. W. Lindenburg and T. Hankemeier, Molecular Biotechnology, Vol. 41, 2009)

Spis treści

Contents Preface .............................................................................. Contributions ....................................................................... Analyte oriented 1. Determination of Small Ions with Capillary Electrophoresis and Contactless Conductivity Detection Andreas Zemann, Irene Rohregger, Roland Zitturi 2. Metal Analysis with Capillary Zone Electrophoresis Ashok Kumar Malik 3. Measurement of Low Molecular Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillary Electrophoresis Ewa Dabek-Zlotorzynska and Valbona Celo 4. Determination of Aliphatic Low Molecular Weight and Biogenic Amines by Capillary Zone Electrophoresis Agnes Fekete, Majlinda R. Lahaniatis, Jutta Lintelmann and Philippe Schmitt-Kopplin 5. Capillary Electrophoretic Analysis of Organic Pollutants Ashok Kumar Malik, Jatinder Singh Aulakh and Varinder Kaur 6. Capillary Electrophoresis with Laser-Induced Fluorescence: Environmental Applications Lee Riddick and William C. Brumley 7. Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules with Capillary Electrophoresis/Mass Spectrometry Moritz Frommberger, Matthias Englmann and Philippe Schmitt-Kopplin 8. Analysis of the Enantiomers of Chiral Pesticides and Other Pollutants in Environmental Samples by Capillary Electrophoresis Arthur W. Garrison, Philippe Schmitt-Kopplin and Jimmy K. Avants 9. Capillary Electrophoresis of Tropane Alkaloids and Glycoalkaloids Occurring in Solanaceae Plants Tommaso R.I. Cataldi and Giuliana Bianco 10. Capillary Electrophoresis for Pharmaceutical Analysis Alex Marsh, Margo Broderick, Kevin Altria, Joe Power, Sheila Donegan and Brian Clark 11. Capillary electrophoresis of Neutral Carbohydrates: Mono-, Oligosaccharides andGlycosides Cristiana Campa and Marco Rossi 12. Capillary Electrophoresis of Sugar Acids Cristiana Campa, Edi Baiutti and Anna Flamigni 13. Use of Capillary Electrophoresis for Polysaccharide Studies and Applications Amelia Gamini, Anna Coslovi, Isabella Rustighi, Cristiana Campa, Amedeo Vetere and Sergio Paoletti 14. Analysis of Oligonucleotides using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry An Willems, Dieter L. Deforce and Jan Van Bocxlaer 15. Separation of DNA by Capillary Electrophoresis Bruce McCord, Brittany Hartzell-Baguley and Stephanie King 16. Capillary Electrophoresis of DNA Damage Guowang Xu, Xianzhe Shi, Chunxia Zhao, Kailong Yuan, Qianfeng Wenig, Peng Gao and Jing Tian 17. Capillary Electrophoresis of Gene Mutation Guowang Xu, Xianzhe Shi, Chunxia Zhao, Kailong Yuan, Qianfeng Wenig, Peng Gao and Jing Tian 18. Biomedical Application of Amino Acid Detection by Capillary Electrophoresis Giuseppe E. De Benedetto 19. Separation of Peptides by Capillary Electrophoresis Gerhard K. E. Scriba and Arndt Psurek 20. Analysis of Proteins by Capillary Electrophoresis Christian W. Huck and Günther K. Bonn 21. Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques Hervé Cottet and Pierre Gareil 22. Capillary Electrophoresis Separation of Microorganisms Bartolomé M. Simonet, Angel Ríos and Miguel Valcárcel Methods oriented 23. A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis Philippe Schmitt-Kopplin and Agnes Fekete 24. The CE-Way of Thinking: 'All is Relative!' Philippe Schmitt-Kopplin and Agnes Fekete 25. Adsorbed Cationic Polymer Coatings for