1 Before the analysis. Rules for interpretation of hepatic cytology1.1 The rules for cytological diagnosis of hepatic diseases1.1.1 Rule 11.1.2 Rule 21.1.3 Rule 31.1.4 Rule 41.1.5 Rule 51.1.6 Rule 61.1.7 Rule 71.2 Diagnostic approach to liver disease1.2.1 Clinical and anamnestic signs1.2.2 Hematochemical investigations1.2.2.1 Pathological bases of liver damage1.2.2.2 Diagnosis of liver damage1.2.2.3 Useful enzymes for the recognition of damage to the hepatocyte and cholangiocyte1.2.2.4 Liver failure diagnosis1.2.2.5 Parameters of liver failure1.2.3 Ultrasonographic investigation1.2.4 Cytological and histopathological investigation1.2.4.1 Sample collection1.2.4.2 Cytological approach to hepatic diseases1.3 To rimember2 Normal Histology and Cytology of the liver2.1 Normal liver histology2.2 Normal cytology of the liver2.2.1 Hepatocytes2.2.2 Kupffer cells2.2.3 Stellate cells2.2.4 Cholangiocytes (biliary cells)2.2.6 Hepatic mast cells2.2.7 Hematopoietic cells2.2.8 Mesothelial cells2.3 To rimember3 Non-specific and reversible hepatocellular damage3.1 Accumulation of water3.2 Accumulation of glycogen3.3 Accumulation of lipids3.4 Accumulation of bilirubin and bile salts3.5 Hyperplasia of stellate cells3.6 Regenerative changes3.7 To rimember4 Intra and extra-cytoplasmic pathological accumulation4.1 Pathological intracytoplasmic accumulation4.1.1. Lipofuscin4.1.2 Copper4.1.3 Iron and hemosiderin4.1.4 Protein droplets4.1.5 Cytoplasmic granular eosinophilic material4.1.6. Hepatic lysosomal storage disorders4.2 Pathological extracytoplasmic accumulations4.2.1 Bile4.2.2 Amyloid4.3 To remember5 Irreversible hepatocellular damage5.1 Necrosis5.2 Apoptosis5.3 To remember6 Inflammation6.1 Presence of neutrophilic granulocyte6.2 Presence of eosinophilic granulocytes6.3 Presence of lymphocytes and plasmacells6.4 Presence of macrophages6.5 Presence of mast cells6.6 To remember7 Nuclear inclusions7.1 "Brick" inclusions7.2 Glycogen pseudo-inclusions7.3 Lead inclusions7.4 Viral inclusions7.5 To remember8 Cytological features of liver fibrosis8.1 Cytological features of liver fibrosis8.2 To remember9 Cytological Features of biliary diseases9.1 General features of biliary diseases9.2 Cytological features of some specific biliary diseases9.2.1 Acute and chronic cholestasis9.2.2 Acute cholangitis9.2.3 Chronic cholangitis9.2.4 Lymphocytic cholangitis9.3 To rimember10 Bile and gallbladder diseases10.1 Bactibilia and septic cholecystitis10.2 Epithelial hyperplasia10.3 Gallbladder mucocele10.4 Limy bile syndrome10.5 Biliary sludge10.6 Neoplastic diseases of gallbladder10.7 Other gallbladder diseases10.8 To remember11 Ethiological agents11.1 Virus11.2 Bacteria11.3 Protozoa11.4 Fungi11.5 Parasites11.6 To remember12 Neoplastic lesions of the hepatic parenchyma12. 2 Nodular lesions of epithelial origins12.2.1 Nodular hyperplasia12.2.2 Hepatocellular adenoma12.2.3 Hepatocellular carcinoma12.2.4 Cholangioma12.2.5 Cholangiocellular carcinoma12.2.6 Other nodular lesions of biliary origins12.2.7 Hepatic carcinoid12.2.8 Hepatoblastoma12.3 Nodular lesions of mesenchymal origin12.3.1 Malignant mesenchymal neoplasms12.4 Nodular lesions of hematopoietic origin12.4.1. Myelolipoma12.4.2. Large-cell hepatic lymphoma12.4.3 Small cell lymphoma12.4.4. LGL lymphoma12.4.5 Epitheliotropic lymphoma12.4.6 Other types of hepatic lymphoma12.4.7. Malignant histiocytic neoplasms12.4.8. Mast cell tumor12.4.9 Hepatic splenosis12.5 Liver metastasis12.6 Criteria for the selection of sampling methods of liver nodular lesions12.7 To remember
Carlo Masserdotti, DVM, Dipl ECVCP, Spec Bioch Clin IAT, Consultant, Anatomic and Clinical Pathology, IDEXX Laboratories, Brescia, Italy.