Candida Species: Methods and Protocols

ISBN-13: 9781071625484 / Angielski / Twarda / 2022

Calderone, Richard

Opis

This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Candida Species: Methods and Protocols, Second Edition serves as an ideal guide for researchers working to further our understanding of this family of infective fungi.

Spis treści

Part I: The Candida Genome

 

1. CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans

            Ben A. Evans and Douglas A. Bernstein

 

2. Plasmid-Based CRISPR–Cas9 Editing in Multiple Candida Species

            Lisa Lombardi and Geraldine Butler

 

3. A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans

            Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan

 

4. How to Use the Candida Genome Database

            Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock

 

5. Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata

            Eunice López-Fuentes, Grecia Hernández-Hernández, Alejandro De Las Peñas, and Irene Castaño

 

6. Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans

            Virginia Glazer and Damian Krysan

 

Part II: Antifungal Discovery and Resistance

 

7. Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance

            Jane Usher

 

8. Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS)

            Jessica Regan and Glen E. Palmer

 

9. High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis

            Mohit Kumar, Rajendra Prasad, and Ashutosh Singh

 

10. Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous     Saccharomyces cerevisiae Mutant Library

            Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She

 

11. Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans

            Dongmei Li, Xiaodong She, and Richard Calderone

 

Part III: Pathogenesis and Virulence

 

12. In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin

            Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik

 

13. Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors

            Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck

 

14. Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy

            Junko Yano and Paul L. Fidel, Jr.

 

15. A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells

            Priya Uppuluri

 

16. A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions

            Priya Uppuluri

 

17. Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract

            Daniel Prieto and Jesús Pla

 

18. Detection and Identification of Candida auris from Clinical Skin Swabs

            Milena Kordalewska and David S. Perlin

 

19. Production and Isolation of the Candida Species Biofilm Extracellular Matrix

            Marienella Hereidia and David Andes

 

Part IV: Communities

 

20. Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model

            Jesús A. Romo and Carol A. Kumamoto

 

21. Analysis of Gut Microbiome Using Fecal Samples

            Ahmed Gamal, Mayyadah Alabdely, Kelly Steller, Hilmi Al-Shakhir, Mohammed Elshaer, and Mahmoud Ghannoum

 

22. Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry

            Dongmei Li, Jie Cheng, Richard Calderone, and Joseph A. Bellanti

 

Part V: Immunity

 

23. Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure

            Ebrima Bojang, Rebecca Drummond, and Rebecca A. Hall

 

24. Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans

            Michael D. Kruppa, Douglas W. Lowman, Harry E. Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V. Hall, Tammy R. Ozment, and David L. Williams

 

25. Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis

            Jessica Saul-McBeth and Heather R. Conti