Cancer Stem Cells: Methods and Protocols

ISBN-13: 9781588299383 / Angielski / Twarda / 2009 / 284 str.

Yu, John S.

Opis

Cancer is a devastating disease that affects millions of people in the world. Our the- pies for tumors have mostly been based on classical chemotherapy and antiproliferative treatments. More recently, directed therapies against a causative oncogene have led to prominent reduction in tumor rates for some cancers. For instance, chronic myelogenous leukemia that is due to a BCR ABL translocation can be targeted with Gleevec. Despite these advances in individual tumors, a number of patients are treated for their primary tumors and ultimately relapse. Relapsing tumors can be due to resistance to chemotherapy or to antioncogene drugs. Another hypothesis is that heterogeneity in the tumor leads to an inability of classical chemotherapy to completely eradicate all cells of the tumor. This concept of heterogeneity has led some investigators to propose a cancer stem cell model. As patients are treated with chemotherapy, most of the dividing cells are killed, but this leaves a small subset of cells that have the ability to remake the entire tumor. These are cancer stem cells. They possess the signals of self-renewal and yet can also differentiate. If one could understand more about the cells that remain after classical chemotherapy or the cells that can remake the tumor among the heterogeneous population, this information would have a huge impact on our treatment of cancer."

Recenzje

From the reviews:

"This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. ... The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. ... The topic is fascinating and clinically relevant." (Bruce A. Fenderson, Doody's Review Service, June, 2010)

Spis treści

1. Defining Cancer Stem Cells by Xenotransplantation in Zebrafish Michael C. Dovey and Leonard I. Zon 2. Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models Yibin Kang 3. Identification of Murine and Human Acute Myeloid Leukemia Stem Cells Aniruddha J. Deshpande, Farid Ahmed, and Christian Buske 4. Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal Ichiro Nakano and Harley I. Kornblum 5. Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133 Kyeung Min Joo and Do-Hyun Nam 6. Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells Hugo Guerrero-Cázares, Kaisorn L. Chaichana, and Alfredo Quiñones-Hinojosa 7. Methodologies in Assaying Prostate Cancer Stem Cells Hangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis, Simon W. Hayward, and Dean G. Tang 8. Characterization of Non-Malignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population Method Murielle Mimeault and Surinder K. Batra 9. Anchorage Independent Growth of Prostate Cancer Stem Cells Shunyou Wang 10. Identification of Human Pancreatic Cancer Stem Cells Chenwei Li, C.J. Lee, and Diane M. Simeone 11. Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma Laurie Ailles and Mark Prince 12. Pituitary Adenoma Stem Cells Patrizia Tunici and John S. Yu 13. Genome-Wide DNA Methylation Profiling: The mDIP-Chip Technology Yin Shen, Shaun D. Fouse, and Guoping Fan 14. The Contribution of Niche-Derived Factors to the Regulation of Cancer Cells Julie B. Sneddon 15. Dendritic Cell Vaccines for Cancer Stem Cells Serena Pellegatta and GaetanoFinocchiaro 16. Maintaining and Engineering Neural Stem Cells for Delivery of Genetically Encoded Therapy to Brain Tumors Jennifer Katz, Joseph Krueger, Brunhilde Felding-Habermann, and Evan Y. Snyder 17. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells Vera S. Donnenberg, E. Michael Meyer, and Albert D. Donnenberg

Blurb

Through the revolutionary concept of cancer stem cells, cancer research has been reinvigorated to study the role of these unique cells in cancer propagation and as targets of innovative therapies. In Cancer Stem Cells: Methods and Protocols, preeminent researchers have compiled cancer stem cell research techniques and protocols to promote healthy competition, discourse, and collaboration in this vital field.  The volume covers extensive topics such as identification and isolation of cancer stem cells, animal models of cancer stem cells, methylation profiling, the contribution of the niche in the regulation of cancer stem cells, immunologic targeting, and the use of normal stem cells as a treatment, among other subjects.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Cancer Stem Cells: Methods and Protocols will aid scientists around the world in the furthering of our understanding of cancer initiation and propagation and, most importantly, in the development of novel targets for cancer therapy.