Cancer Immunotherapy: Methods and Protocols

ISBN-13: 9781071635926 / Angielski

Velia Siciliano; Francesca Ceroni

Spis treści

1. Isolation of live immune cells from the tumour microenvironment by FACS

Aikaterini Kafka, Christos Ermogenous, and Luigi Ombrato

 

2. In vitro evaluation of cancer cell immunogenicity and antigen-specific T-cell cytotoxicity by flow cytometry

Martina Musella, Nicoletta Manduca, Ester Maccafeo, Eliana Ruggiero, and Antonella Sistigu

 3. Retroviral transduction of human primary T cells followed by real-time T-cell mediated cancer cell cytolysis analysis

Anne Rahbec, Reno Debets, Per thor Straten and Marlies J.W. Peeters

 

4. Expansion and retroviral transduction of primary murine T cells for CAR T cell therapy

Pauline Loos, Lauralie Short, Gillian Savage, and Laura Evgin

 

5. In Situ Decellularisation of Tissues applied to the topographical analysis of tumour associated extracellular matrix

Alejandro E Mayorca Guiliani

 

6. Monitoring cell cytoskeleton variations upon piezoelectric stimulation: Implications for the immune system

Özlem Şen, Carlotta Pucci, and Gianni Ciofani

 

7. Preparation method and in vitro characterization of nanoparticles sensitive to tumor microenvironment

Naym Blal and Daniela Guarnieri

 

8. A new microfluidic device to facilitate functional precision medicine assays

Albert Manzano-Muñoz, Jose Yeste, María A. Ortega, Josep Samitier, Javier Ramón-Azcón, and Joan Montero

 

9. Kinetic detection of apoptosis events via caspase 3/7 activation in a tumor-immune microenvironment on a chip

Bertani F.R., Dabbagh Moghaddam F., Panella C., Giannitelli S.M., Peluzzi V., Gerardino A., Rainer A., Roscilli G.​, De Ninno A. and Businaro L. 

 

10. Hypoxic 3D Tumor Model for Evaluating of CAR-T Cell Therapy In Vitro

Jeong Min Oh and Keyue Shen

 

11. Rapid screening of CAR T cell functional improvement strategies by highly-multiplexed single-cell secretomics

Dragana Slavkovic-Lukic, Jessica Fioravanti, Azucena Martín-Santos, Edward Han, Jing Zhou, and  Luca Gattinoni

 

12. Genome editing in CAR-T cells using CRISPR/Cas9 technology

Irene Andreu-Saumell, Alba Rodriguez-Garcia, and Sonia Guedan

13. Genetic modification of tumor-infiltrating lymphocytes, peripheral T cells, and T cell model cell lines

Hadas Weinstein-Marom, Dayana Blokon-Kogan, Maya Levi- Mann, Chaja Katzman, Shira Shalev, Masha Zaitsev, Michal J. Besser, Ronnie Shapira-Frommer, Gideon Gross, Orit Itzhaki, and Lior Nissim

 

14. Transposon-based manufacturing of human CAR-T cells

¹Megan Tennant and  Richard O’Neil

 

15. Redirecting human conventional and regulatory T cells using chimeric antigen receptors

Capers M. Zimmerman, Rob A. Robino, Russell W. Cochrane, Matthew D. Dominguez, and Leonardo M.R. Ferreira

 

16. How to Test Human CAR T cells in Solid Tumors, The Next Frontier of CAR T Cell Therapy

Russell W. Cochrane,Andrew Fiorentino, Eva Allen, Rob A. Robino, Jaime Quiroga, and Leonardo M.R. Ferreira

 

17. Nano-Optogenetic CAR-T Cell Immunotherapy

Nhung Thi Nguyen, Siyao Liu, Gang Han, Yubin Zhou, and Kai Huang

 

18. A non-viral piggyBac transposon-mediated method to generate large-scale CAR-NK cells from human peripheral blood primary NK cells

Zhicheng Du, Tianzhi Zhao , Xianjin Chen , Shijun Zha  and Shu Wang

 

19. Engineering probiotic E.coli Nissle 1917 for release of therapeutic nanobodies

Candice Gurbatri and Tal Danino

Blurb

This volume details multiple areas of new and emerging methods to develop the next generation of immunotherapy treatments. Chapters guide readers through analysis and characterisation of the interactions between tumour and immune cells, and cell engineering tools for cancer treatment, to provide a unique and compelling set of techniques instrumental to work with, and engineer, immune cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Cancer Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.