Cancer Immunosurveillance: Methods and Protocols

ISBN-13: 9781493988846 / Angielski / Twarda / 2018 / 363 str.

Alejandro Lopez-Soto; Alicia Folgueras

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer
Willem H. Zoutman, Rogier J. Nell, and Pieter A. van der Velden

2. An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood
Imran Uddin, Kroopa Joshi, Theres Oakes, James M. Heather, Charles Swanton on behalf of the TRACERx consortiumx, and Benny Chain

3. Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells
Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, and Jianxun Song

4. Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Anti-Tumor Effects In Vivo
Akshat Sharma, Nicholas A. Zumwalde, and Jenny E. Gumperz

5. Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors
Yong Yu and Pentao Liu

6. Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance
Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega,  and Massimo Vitale

7. CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor ActivitySeila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, and Alejandro López-Soto

8. A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro
Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, and Alejandro López-Soto

9. Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency
Vidhya R. Nair and Srinivas Malladi

10. Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity
Nicholas M. Clark and Paula D. Bos

11. Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo
Magali Dupont and Christian A.J. Vosshenrich

12. Determination and Isolation of Immune Populations from Brain Tumor Microenvironments
Javier Godino and Alberto J. Schuhmacher

13. Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells
Llipsy Santiago, Marta Castro, Julián Pardo, and Maykel Arias

14. Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry
Shelly Kalaora and Yardena Samuels

15. Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways
Cherie’ R. Scurrah, Alan J. Simmons, and Ken S. Lau

16. Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation
Marina Wierz, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, and Jerome Paggetti

17. Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion
Chiara Nicolazzo, Angela Gradilone, Guido Carpino, Paola Gazzaniga, and Cristina Raimondi

18. Quantitative Identification of Senescent Cells in Cancer
Anat Biran, Ziv Porat, and Valery Krizhanovsky

19. Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance
Dirk Zboralski and Anna Frömming

20. Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment
Xiuyun Jiang, Y. David Seo, Kevin Sullivan, and Venu G. Pillarisetty

21. Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models
Juliette Humeau, Sarah Lévesque, Guido Kroemer, and Jonathan G. Pol

22. A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers
Annunziata Nigro, Barbara Montico, Vincenzo Casolaro, and Jessica Dal Col

23. Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy
Yuan He, Wijnand Helfrich, and Edwin Bremer

24. Generation of CAR-T Cells for Cancer Immunotherapy
Qumiao Xu, Hizkia Harto, Robert Berahovich, Shirley Xu, Hua Zhou, Vita Golubovskaya, and Lijun Wu

Blurb

This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field.