Cancer Genomics and Proteomics: Methods and Protocols

ISBN-13: 9781493946549 / Angielski / Miękka / 2016 / 262 str.

Narendra Wajapeyee

Opis

Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cancer Genomics and Proteomics: Methods and Protocols, Second Edition seeks to aid scientists in the further study into various aspects of tumor initiation and progression.

Spis treści

1. Genome-Wide Mapping Of RNA Pol-II Promoter Usage In Mouse Tissues By Chip-Seq

Sharmistha Pal, Ravi Gupta And Ramana V. Davuluri

 

2. Using Native Chromatin Immunoprecipitation To Interrogate Histone Variant Protein Deposition In Embryonic Stem Cells

Zito Tseng, Tao Wu, Yifei Liu, Mei Zhong, And Andrew Xiao

 

3. Reduced Representation Bisulfite Sequencing To Identify Global Alteration In The DNA Methylation

Arvindhan Nagarajan, Christine Roden And Narendra Wajapeyee

 

4. A High-Throughput Microrna Expression Profiling System

Yanwen Guo, Stephen Mastriano And Jun Lu

 

5. Using Pooled Mir30-Shrna Library For Cancer Lethal And Synthetic Lethal Screens

Liam Changwoo Lee, Shaojian Gao, Qiuning Li, And Ji Luo

 

6. Genome-Wide RNA Interference Screening To Identify Regulators Of Epigenetic Silencing Of A Tumor Suppressor Gene

Matteo Forloni, Thuy Ho And Narendra Wajapeyee

 

7. A Diphtheria Toxin Negative Selection In RNA Interference Screening

Zhi Sheng, Susan F Murphy, Sujuan Guo And Michael R Green

 

8. Cancer Metabolism: Crosstalk Between Signaling And O-Glcnacylation

Christina M. Ferrer And Mauricio J. Reginato

 

9. Targeted Genome Modification Via Triple Helix Formation

Adele S. Ricciardi, Nicole A. Mcneer, Kavitha K. Anandalingam, W. Mark Saltzman, Peter M. Glazer

 

10. Synthesis Of Stabilized Alpha-Helical Peptides

Federico Bernal And Samuel G. Katz

 

11. Arginine-Grafted Biodegradable Polymer: A Versatile Transfection Reagent For Both DNA And Sirna

Jagadish Beloor, Hye Yeong Nam, Sang-Kyung Lee And Priti Kumar

 

12. Using Laco Arrays To Monitor DNA Double-Strand Break Dynamics In Live Schizosaccharomyces Pombe Cells

Bryan A. Leland And Megan C. King

 

13. Zebrafish As A Platform To Study Tumor Progression

Corrie A. Painter And Craig J. Ceol

14. Clonal Screens To Find Modifiers Of Partially Penetrant Phenotypes In C. Elegans

Michael E. Hurwitz

 

15. Serum Profiling Using Protein Microarrays To Identify Disease Related Antigens.

Donald Sharon And Michael Snyder

 

16. Interrogation Of In Vivo Protein-Protein Interactions Using Transgenic Mouse Models And Stable Isotope Labeling

Anwesha Dey, Jiansheng Wu, Donald S. Kirkpatrick

 

17. New Biophysical Methods To Study The Membrane Activity Of Bcl-2 Proteins

Stephanie Bleicken And Ana J. Garcia-Saez

 

18. Purification Of Recombinant 2XMBP Tagged Human Proteins From Human Cells

Ryan Jensen

 

19. Computational Analysis In Cancer Exome Sequencing

Perry Evans, Yong Kong, And Michael Krauthammer

 

20. Matrix Factorization Methods For Integrative Cancer Genomics

Shihua Zhang And Xianghong Jasmine Zhou

 

21. Computational Methods For DNA Copy-Number Analysis Of Tumors

Jude Kendall And Alexander Krasnitz