Cancer Gene Profiling: Methods and Protocols

ISBN-13: 9781493979974 / Angielski / Miękka / 2019 / 256 str.

Robert Grutzmann;Christian Pilarsky

Opis

This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second Edition explores different techniques and approaches needed to understand the key stages of cancer development, as using only one technique would be insufficient. This book provides readers with an overview of the state-of-the-art methods that will enable them to perform these experiments, and demonstrates the kind of analysis that is possible in our current biomedical research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Cancer Gene Profiling: Methods and Protocols, Second Edition is a great resource for any student or practitioner with an interest in cancer gene profiling, and can be used in any well-equipped research laboratory.

Spis treści

1. Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository
Daniel S. Atherton, Katherine C. Sexton, Dennis Otali, Walter C. Bell, and William E. Grizzle

2. Tissue Microdissection
Anja Rabien and Glen Kristiansen

3. Tissue Microarrays
Ana-Maria Dancau, Ronald Simon, Martina Mirlacher, and Guido Sauter

4. Gene Expression Analysis in the Age of Mass Sequencing: An Introduction
Christian Pilarsky, Lahiri Kanth Nanduri, and Janine Roy

5. Quantitative DNA Methylation in Cancer
Ole Ammerpohl, Andrea Haake, Julia Kolarova, and Reiner Siebert

6. Single Nucleotide Polymorphism to Associate Cancer Risk
Victoria Shaw, Katie Bullock, and William Greenhalf

7: Exosomes in Cancer Disease
Margot Zöller

8: MicroRNAs (miRNAs) Profiling
Lu Gao and Feng Jiang

9. Cancer Gene Profiling for Response Prediction
B. Michael Ghadimi and Peter Jo

10. Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment
Sanjay Tiwari, Ole Ammerpohl, and Holger Kalthoff

11. Application of Proteomics in Cancer Biomarker Discovery: GeLC-MS/MS
Pedro R. Cutillas and Tatjana Crnogorac-Jurcevic

12. Meta-Analysis of Cancer Gene Profiling Data
Janine Roy, Christof Winter, and Michael Schroeder

13. Reproducible, Scalable Fusion Gene Detection from RNA-Seq
Vladan Arsenijevic and Brandi N. Davis-Dusenbery

14. Transcriptome Sequencing for the Detection of Chimeric Transcripts
Hsueh-Ting Chu