Cancer Drug Resistance: Overviews and Methods

ISBN-13: 9781493933457 / Angielski / Twarda / 2016 / 314 str.

Jose Rueff; Antonio Sebastiao Rodrigues

Opis

This volume discusses the latest advancements and technologies used in cancer drug resistance research. Cancer Drug Resistance: Overviews and Methodscontains chapters that cover topics such as: studying the mechanics of resistance to DNA damaging therapeutic drugs; studies to delineate the role of efflux transporters; expression of drug transporters; resistance to targeted therapies in breast cancer; the role of microRNAs in current pancreatic cancer treatment; and cancer exosomes as mediators of drug resistance or clinical and molecular methods in drug development and the use of bioinformatics in the management of cancer drug resistance data. Written in the highly successfulMethods in Molecular Biology series format, chapters include overviews of the main issues in cancer drug resistance and the respective mechanisms, as well as introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Drug Resistance: Overviews and Methods, is a valuable resource to researchers, oncobiologists and clinical oncologists or anyone else who is interested in the study of cancer and its drug resistances."

Spis treści

1. Cancer Drug Resistance: A Brief Overview from a Genetic Viewpoint 
José Rueff and António S. Rodrigues

2. Classical and Targeted Anti-Cancer Drugs: An Appraisal of Mechanisms of Multidrug Resistance
Bruce C. Baguley

3. In Vitro Methods for Studying the Mechanisms of Resistance to DNA Damaging Therapeutic Drugs
Pasarat Khongkow, Anna

K. Middleton, Jocelyn P.-M. Wong,­ Navrohit K. Kandola, Mesayamas Kongsema, Gabriela Nestal de Moraes, Ana R. Gomes, and Eric W.-F. Lam

4. In Vitro Approaches to Study Regulation of Hepatic Cytochrome P450 (CYP) 3A Expression by Paclitaxel and Rifampicin
Romi Ghose, Pankajini Mallick, Guncha Taneja, Chun Chu, and Bhagavatula Moorthy

5. Uptake and Permeability Studies to Delineate the Role of Efflux Transporters
Ramya Krishna Vadlapatla, Dhananjay Pal, and Ashim K. Mitra

6. Dynamics of Expression of Drug Transporters: Methods for Appraisal
Marta Gromicho, José Rueff, and António S. Rodrigues

7. Fluorimetric Methods for Analysis of Permeability, Drug Transport Kinetics, and Inhibition of the ABCB1 Membrane Transporter
Ana Armada, Célia Martins, Gabriella Spengler, Joseph Molnar, Leonard Amaral, António S. Rodrigues, and Miguel Viveiros

8. Resistance to Targeted Therapies in Breast Cancer
Sofia Braga

9. MicroRNAs and Cancer Drug Resistance
Bruno Costa Gomes, José Rueff, and António S. Rodrigues

10. The Role of MicroRNAs in Resistance to Current Pancreatic Cancer Treatment: Translational Studies and Basic Protocols for Extraction and PCR Analysis
Ingrid Garajová, Tessa Y.S. Le Large, Elisa Giovannetti, Geert Kazemier, Guido Biasco, and Godefridus J. Peters

11. Methods for Studying MicroRNAs Expression and Their Targets in Formalin-Fixed, Paraffin-Embedded (FFPE) Breast Cancer Tissues
Bruno Costa Gomes, Bruno Santos, José Rueff, and António S. Rodrigues

12. The Regulatory Role of Long Non-Coding RNAs in Cancer Drug Resistance
Marjan E. Askarian-Amiri, Euphemia Leung, Graeme Finlay, and Bruce C. Baguley

13. Cancer Exosomes as Mediators of Drug Resistance
Maria do Rosário André, Ana Pedro, and David Lyden

14. Isolation and Characterization of Cancer Stem Cells from Primary Head and Neck Squamous Cell Carcinoma Tumors
Hong S. Kim, Alexander T. Pearson, and Jacques E. Nör

15. Clinical and Molecular Methods in Drug Development: Neoadjuvant Systemic Therapy in Breast Cancer as a Model
Sofia Braga

16. Proteomics in the Assessment of the Therapeutic Response of Anti-Neoplastic Drugs: Strategies and Practical Applications
Vukosava Milic Torres, Lazar Popovic, Fátima Vaz, and Deborah Penque

17. Managing Drug Resistance in Cancer: Role of Cancer Informatics
Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, Sudheer Gupta, Harinder Singh, and Gajendra P.S. Raghava