Cancer Chemoprevention: Methods and Protocols

ISBN-13: 9781493949243 / Angielski / Miękka / 2016 / 214 str.

Sabrina Strano

Spis treści

1. Controlled Delivery of Chemopreventive Agents by Polymeric Implants

Farrukh Aqil and Ramesh C. Gupta

 

2. Use Of Buffy Coat Mirna Profiling for Breast Cancer Prediction in Health Women

Sara Donzelli ,Giovanni Blandino, and Paola Muti

 

3. Micrornas in Cancer Chemoprevention: Method to Isolate them from Fresh Tissues

Federica Ganci and Giovanni Blandino

 

4. Application of RNA-Seq Technology in Cancer Chemoprevention

Frauke Goeman and Maurizio Fanciulli

 

5. Detection of Circulating Tumor DNA in the Blood of Cancer Patients: An Important Tool in Cancer Chemoprevention

Peter Ulz, Martina Auer, and Ellen Heitzer

 

6. The Methylated DNA Immunoprecipitation (Medip) to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development.

Silvia Masciarelli, Teresa Bellissimo, Ilaria Iosue, and Francesco Fazi

 

7. LC-MS-Based Metabolomic Investigation Of ChemopreventivePhytochemical-Elicited Metabolic Events

Lei Wang, Dan Yao,and  Chi Chen

 

8.¹H NMR Metabolomic Footprinting Analysis for the In Vitro Screening of Potential Chemopreventive Agents

Luca Casadei and Mariacristina Valerio

 

9. Comet Assay in Cancer Chemoprevention

Raffaela Santoro, Maria Ferraiuolo, Gian Paolo Morgano, Paola Muti, and Sabrina Strano

 

10. Angiogenesis Assays

     Dhanya K. Nambiar, Praveen K. Kujur, and Rana P. Singh

 

11. " Algimatrix^tm Based 3D Cell Culture System as an In-Vitro Tumor Model: an   Important Tool In Cancer Research"

 Chandraiah Godugu and Mandip  Singh

 

12. Cancer Gastric Chemoprevention: Isolation Of Gastric Tumor Initiating Cells

Federica

Mori^, Valeria Canu, Laura Lorenzon, Alfredo Garofalo, Giovanni Blandino, and Sabrina Strano

 

13. Isolation of Chemoresistance Cell Subpopulations

Claudia Canino And Mario Cioce

 

14.Autophagy In Cancer Chemoprevention: Identification of Novel  Autophagy Modulators with Anticancer Potential

Yuanzhi Lao and Naihan Xu

 

 15. Protocol for a Steady-State FRET Assay in Cancer Chemoprevention

Marjolein C. A. Schaap, Andreia M. R. Guimarães, Andrew F. Wilderspin, and Geoffrey Wells

 

16. 3D Tumour Models and Time –Lapse Analysis by Multi-Dimensional Microscopy

Dimitri Scholz and Nobue Itasaki

 

17. Antibody Array as a Tool for Screening of Natural Agents in Cancer Chemoprevention

Claudio Pulito, Andrea Sacconi , Anna Maidecchi, Etleva Korita,  and Sabrina Strano

 

18. South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anti-Cancer Splicing Activity

Zodwa Dlamini, Zukile Mbita,  and David Bates

Blurb

This volume presents state of the art of methods that can be useful for both basic and translational researchers to conduct chemoprevention preclinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Cancer Chemoprevention: Methods and Protocols aims to ensure successful results in the further study of this vital field.