Cancer Cell Culture: Methods and Protocols

ISBN-13: 9781493956579 / Angielski / Miękka / 2016 / 502 str.

Ian A. Cree

Opis

This new edition covers the recent advances in cancer cell culture research, including novel cell culture procedures. It includes methods of authenticating cell lines, functional assays to evaluate clonogenicity, and stresses safe working practice throughout.

Spis treści

1. Cancer Biology

            Ian A. Cree

 

2. Principles of Cancer Cell Culture

            Ian A. Cree

 

3. Storage of Cell Lines

            Katharine A. Parker

 

4. Characterization and Authentication of Cancer Cell Lines: An Overview

            Yvonne A. Reid

 

5. Online Verification of Human Cell Line Identity by STR DNA Typing

            Wilhelm G. Dirks and Hans G. Drexler

 

6. Cytogenetic Analysis of Cancer Cell Lines

            Roderick A. F. MacLeod and Hans G. Drexler

 

7. Cell Culture Contamination

            Glyn N. Stacey

 

8. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction

            Cord C. Uphoff and Hans G. Drexler

 

9. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics

            Cord C. Uphoff and Hans G. Drexler

 

10. Quality Assurance and Good Laboratory Practice

            Louise A. Knight and Ian A. Cree

 

11. Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning

            Laura Breen, Joanne Keenan, and Martin Clynes

 

12. Isolation and Culture of Colon Cancer Cells and Cell Lines

            Sharon Glaysher and Ian A. Cree

 

13. Isolation and Culture of Melanoma and Naevus Cells and Cell Lines

            Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett

 

14. Isolation and Culture of Squamous Cell Carcinomalines

            Karin J. Purdie, Celine Pourreyron, and Andrew P. South

 

15. Isolation and Culture of Ovarian Cancer Cells and Cell Lines

            Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz

 

16. Establishment and Culture of Leukemia-Lymphoma Cell Lines

            Hans G. Drexler

 

17. Isolation of Inflammatory Cells from Human Tumors

            Marta Polak

 

18. Isolation of Endothelial Cells from Human Tumors

            Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, Roland S. Croner, and Michael Stürzl

 

19. Cellular Chemosensitivity Assays: An Overview

            Venil N. Sumantran

 

20. Cell Sensitivity Assays: The MTT Assay

            J. van Meerlo, G.J.L. Kaspers, and J.Cloos

 

21. Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay

            Sharon Glaysher and Ian A. Cree

 

22. The Differential Staining Cytotoxicity (DiSC) Assay: A Review

            Larry M. Weisenthal

 

23. Real-time Cytotoxicity Assays

            Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, and Jonathan M. Cooper

 

24. FPurification of Annexin V and its use in the Detection of Apoptotic Cells

            Katy M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss

 

25. Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay

            Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley

 

26. Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR

            Björn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod

 

27. Cell Migration and Invasion Assays

            K.A. Moutasim, M.L. Nystrom, and G.J. Thomas

 

28. Angiogenesis Assays

            V. Poulaki

 

29. Flow Cytometric DNA Analysis of Human Cancers and Cell Lines

            Sarah A. Krueger and George D. Wilson

 

30. Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines

            Stefan Nagel and Hans G. Drexler

 

31. Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transciptase Polymerase Chain Reaction

            Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree

 

32. Proteomic Evaluation of Cancer Cells – Identification of Cell Surface Proteins

            Samantha Larkin and Claire Hastie

 

33. Development of Rituximab-resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-mediated Immunotherapy

            Ali R. Jazirehi and Bejamin Bonavida

 

34. High Throughput Screening of Natural Products for Cancer Therapy: A Review

            Alan L. Harvey and Ian A. Cree

 

35. Analysis of Drug Interactions

            Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters

 

36. Transfection and DNA-mediated Gene Transfer

            Davide Zecchin and Federica Di Nicolantonio

 

37. Drug Design and Testing: Profiling of Anti-proliferative Agents for Cancer Therapy Using a Cell-based Methyl-[3H]-Thymidine Incorporation Assay

            Matthew Griffiths and Hardy Sundaram

 

38. Feeder Layers: Co-culture with Non-neoplastic Cells

            Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South

 

39. Xenotransplantation of Breast Cancers

            Massimiliani Cariati, Rebecca Marlow, and Gabriela Dontu

Blurb

With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.