Caenorhabditis Elegans: Cell Biology and Physiology: Volume 107

ISBN-13: 9780123946201 / Angielski / Twarda / 2012 / 512 str.

Joel Rothman

Opis

The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

• Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
• Chapters written by leaders in the field
• Tried and true methods deliver busy researchers a one-stop compendium of essential protocols

Spis treści

1.Imaging

High-resolution Imaging of Cellular Processes in C. elegansImmunofluorescence Microscopy Fluorescent Protein Methods: Strategies and ApplicationsModern Electron Microscopy Methods for C. elegans

2. Cell Biology

Culture and Manipulation of Embryonic CellsLaser Microsurgery in Caenorhabditis elegans Analysis of Cell polarityAnalysis of OrganellesC. elegans Cell Cycle Analysis

3. Physiology

Methods for Studying Programmed Cell Death in C. elegansMethods for Studying the DNA Damage Response in the Caenorhabditis elegans GermlineAnalysis of Aging in Caenorhabditis elegansAnalyses of C. elegans Fat Metabolic PathwaysElectrophysiological Methods for C. elegans Neurobiology