CRISPR-Cas Methods: Volume 2

ISBN-13: 9781071616598 / Angielski / Miękka / 2022

Islam, M. Tofazzal

Opis

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis.   Authoritative and cutting-edge,  CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a  holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.
The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1.     A Complete Methodology for the Instruction of CRISPR-based Gene Editing Using a Simplified Cell-free Extract System with Genetic Readout in Bacteria

Kristen M. Pisarcik, Brett M. Sansbury and Eric B. Kmiec

 

2.     In silico Analysis of gRNA Secondary Structure to Predict Its Efficacy

for Plant Genome Editing

Md Mahmudul Hassan, Abul Kashem Chowdhury and Tofazzal Islam

 

3.     Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants

Yingxiao Zhang and Yiping Qi

 

4.     CRISPR-dCas9 Based Targeted Manipulation of DNA Methylation in Plants

Basudev Ghoshala and Jason Gardinera

 

5.     Rapid Assembly of Multiplex Natural CRISPR Arrays

Robert M. Cooper and Jeff Hasty

 

6.     Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants

Simon Sretenovic, Changtian Pan, and Yiping Qi

 

7.     Designing, Performing and Analysing CRISPR-Cas9-mediated Genome Editing Experiments in Leguminous Plant

Dipak Gayen and Subhasis Karmakar

 

8.     Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9

Li Chen, Yupeng Cai and Wensheng Hou

 

9.     Targeted Base Editing in Soybean using a CRISPR-Cas9 Cytidine Deaminase Fusion

Li Chen, Yupeng Cai and Wensheng Hou

 

10.  Genome Editing in Potato Using a Hairy Root Transformation System

Nathaniel M. Butler and Jiming Jiang

11.  Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato

Viswanathan Satheesh, Jinkai Li and Mingguang Lei

 

12.  CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single and Multi-Target Vector Construction

Hirendra Nath Barman

13.  Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9

Chaolei Liu, Chun Wang and Kejian Wang

 

14.  CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants

Yun-Mu Zhang, Yinong Yang and Kabin Xie

 

15.  CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells

Stijn L.M. in ’t Groen, Mike Broeders, and W.W.M. Pim Pijnappel

 

16.  Cell-Type-Specific CRISPR-Cas9 System with miRNAs

Moe Hirosawa and Hirohide Saito

 

17.  CRISPR-Cas9 gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-puro Vectors

Zubaida Sa’id Ameen, Ece Çakiroglu, Serif Senturk, Abdullahi Umar Ibrahhim and Mehmet Ozsoz

 

18.  An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA that Regulate CRISPR-Cas systems

Ping Lin, Qinqin Pu, and Min Wu 

19.  Genetic Engineering of a Phage-based Delivery System for Endogenous III-A CRISPR-Cas System against Mycobacterium tuberculosis

Li Zheng Jiang, Junwei Wei, Nan Peng and Yingjun Li

 

20.  CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages

Yibao Chen, Xiangmin Li, Shuang Wang, Ping Qian, Yingjun Li

 

21.  CRISPR-Cas Genome Editing in the Cellulolytic Bacterium Clostridium thermocellum

Jiang Sean Stettner and Carrie Eckert

 

22.  Gene Targeting in Caenorhabditis elegans Using a Combination of Multiple sgRNAs and a Homologous Recombination-mediated Repair

Jose Luis Tellez-Arreola

 

23.  Design of Repair Templates for CRISPR-Cas9-triggered Homologous Recombination in Caenorhabditis elegans

Li Hyun-Min Kim and Xiaojuan Zhang