C. elegans: Methods and Applications

ISBN-13: 9781071621837 / Angielski

Gal Haspel; Anne C. Hart

Opis

This detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, Caenorhabditis elegans, a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology and behavior, to individuality and interaction with the environment. Beginning with two chapters offering practical advice for setting up research programs with C. elegans, either for a new research group or for a short-term project such as a teaching laboratory, the volume continues with numerous hands-on methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, C. elegans: Methods and Applications, Third Edition offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms.

Spis treści

1. Starting a C. elegans Research Laboratory: Practical Advice

            Anne C. Hart and Heather. L. Bennett

 

2. The Basics of Setting Up Successful Teaching Labs and Short-Term Projects with C. elegans

            Elizabeth E. Glater

 

3. Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose

            Kevin F. O’Connell

 

4. Genetic Methods for Cellular Manipulation in C. elegans

            Menachem Katz

 

5. Observing and Quantifying Fluorescent Reporters

            Sreeparna Pradhan and Michael Hendricks

 

6. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior

            Amelie Bergs, Thilo Henss, Caspar Glock, Jatin Nagpal, and Alexander Gottschalk

 

7. Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons while Monitoring Feeding Behavior

            Nicholas F. Trojanowski and Christopher Fang-Yen

 

8. Antibody Staining for Nematodes with Heat-Induced Antigen Retrieval (HIAR)

            Curtis M. Loer

 

9. ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy

            Chih-Chieh (Jay) Yu, Danielle M. Orozco Cosio, and Edward S. Boyden

 

10. A Fusion-PCR Method for Expressing Genetic Tools in C. elegans

            Eduard Bokman, Yifat Eliezer, and Alon Zaslaver

 

11. Approaches for CRISPR/Cas9 Genome Editing in C. elegans

            Charlotte J. Martin and John A. Calarco

 

12. Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans

            Sonia El Mouridi and Christian Frøkjær-Jensen

 

13. Mutation Mapping and Identification by Whole-Genome Sequencing

            Harold E. Smith

 

14. Lipid Extraction and Analysis

            Henry H. Harrison and Jennifer L. Watts

 

15. Isolating Caenorhabditis elegans from the Natural Habitat

            Clotilde Gimond, Nausicaa Poullet, and Christian Braendle

 

16. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans

            Ross C. Lagoy, Eric Larsen, Dan Lawler, Hamilton White, and Dirk R. Albrecht

 

17. Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

            Maria B. Harreguy, Tracy S. Tran, and Gal Haspel

 

18. An Imaging System for Monitoring C. elegans Behavior and Aging

            Matthew A. Churgin and Christopher Fang-Yen

 

19. Methods for Modulating and Measuring Neuromuscular Exertion in C. elegans

            Kiley J. Hughes and Andrés G. Vidal-Gadea

 

20. Recording and Quantifying C. elegans Behavior

            Navin Pokala and Steven W. Flavell

 

21. Primer on Mathematical Modeling in C. elegans

            Ayush Ranawade and Erel Levine