Breast Cancer: Methods and Protocols

ISBN-13: 9781493934423 / Angielski / Twarda / 2016 / 332 str.

Jian Cao

Opis

This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.

Spis treści

Part I Detection of Molecular Markers of Breast Cancer

1.       Basic Histopathological Methods and Breast Lesion Types for Research
Nengtai Ouyang and Lin Wang

2.       Clinical Applications for Immunohistochemistry of Breast Lesions
Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu

3.       Immunohistochemistry for Triple-Negative Breast Cancer
Kalnisha Naidoo and Sarah E.

Pinder

4.       In Situ Hybridization of Breast Cancer Markers
Li Min and Chengchao Shou

5.       Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH)
Harry C. Hwang and Allen M. Gown

Part II Genetic Detection for Breast Cancer

6.

       Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR)
Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou

7.       Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer
Martin Brown and Meiyun Fan

8.       Pyrosequencing Analysis for Breast Cancer DNA Methylome
Cem Kuscu and Canan Kuscu

Part III Isolation of Breast Cancer Cells

9.       Vita-Assay ™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs)
Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen

10.   Breast Cancer Stem Cell Isolation
Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell

Part IV In Vitro Experimental Models for Breast Cancer

11.   Cellular Apoptosis Assay of Breast Cancer
Yu Sun and Wei-Xing Zong

12.   Assessment of Matrix Metalloproteinases by Gel Zymography
Jillian Cathcart

13.   Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay
Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang

14.   Determination of Breast Cancer Cell Migratory Ability
David Schmitt, Joel Andrews, and Ming Tan

15.   A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao

16.   Three-Dimensional Assay for Studying Breast Cancer Cell Invasion
Nikki A. Evensen
17.   A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation
Ashleigh Pulkoski-Gross

18.   Analysis of Invadopodia Formation in Breast Cancer Cells
Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du

19.   Patient-Derived Tumor Xenograft Models of Breast Cancer
Christopher D. Suarez and Laurie E. Littlepage

20.   Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors
Maryia Lu Li, Wei Rachel Tay, Jingquan He, and Guangwei Du

21.   3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method
Fransisca Leonard and Biana Godin

V In Vivo Experimental Models for Breast Cancer

22.   Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model
Jacquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks

23.   Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models
Wei Wang, Subhasree Nag, and Ruiwen Zhang

24.   Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells
Derek Rammelkamp, Weiyi Li, and Yizhi Meng

25.   Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression
Gregg B. Fields and Maciej J. Stawikowski

O autorze

JIAN CAO Room 004, Life Sciences Building Division of Cancer Prevention Department of Medicine School of Medicine, Stony Brook University Stony Brook, NY 11794-5200 631-632-1815 email: Jian.Cao@stonybrookmedicine.edu