Brain Development: Methods and Protocols

ISBN-13: 9781493960255 / Angielski / Miękka / 2016 / 317 str.

Simon G. Sprecher

Opis

This book introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models. It provides step-by-step detail essential for reproducible results and expert implementation advice.

Spis treści

Part I: Drosophila Protocols

 

1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain

            Danielle C. Diaper and Frank Hirth

 

2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

            David Jussen and Rolf Urbach

 

3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling

            Karoline F. Kraft and Rolf Urbach

 

4. Flybow to Dissect Circuit Assembly in the Drosophila Brain

            Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker

 

5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe

            Benjamin Perruchoud and Boris Egger

 

6. Using MARCM to Study Drosophila Brain Development

            Gudrun Viktorin

 

Part II: Other Arthropods

 

7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria

            George Boyan and Yu Liu

 

Part III: Molluscs

 

8. Methods in Brain Development of Molluscs

            Andreas Wanninger and Tim Wollesen

 

Part IV: Xenopus Protocols

 

9. In Situ Hybridization and Immunostaining of Xenopus Brain

            Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu

 

10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development

            Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford

 

11. Morpholino Studies in Xenopus Brain Development

            Jennifer E. Bestman and Hollis T. Cline

 

Part V: Zebrafish Protocols

 

12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification

            Gilbert Lauter, Iris Söll, and Giselbert Hauptmann

 

13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish

            Adèle Faucherre and Hernán López-Schier

 

14. Anatomical Dissection of Zebrafish Brain Development

            Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins

 

Part VI: Chicken Protocols

 

15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain

            Richard P. Tucker and Qizhi Gong

 

16. Transplantation of Neural Tissue: Quail-Chick Chimeras

            Andrea Streit and Claudio D. Stern

 

17. RNAi-Based Gene Silencing in Chicken Brain Development

            Irwin Andermatt and Esther T. Stoeckli

 

Part VII: Mouse Protocols

 

18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

            Jinling Liu and Aimin Liu

 

19. In Utero Electroporation to Study Mouse Brain Development

            Emilie Pacary and François Guillemot

 

20. The Cre/Lox System to Assess the Development of the Mouse Brain

            Claudius F. Kratochwil and Filippo M. Rijli

Blurb

Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.