1. Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue
Alan G. Barbour and Vanessa J. Cook
2. Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato using Molecular Biological Methods
Gabriele Margos, Isabell Notter, and Volker Fingerle
3. Generation of Mammalian Host-adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats
Melissa J. Caimano
4. Co-immunoprecipitation for Identifying Protein-protein Interactions in Borrelia Burgdorferi
Henna Iqbal, Darrin R. Akins, and Melisha R. Kenedy
5. Characterization of Borreliaburgdorferi Binding to Mammalian Cells and Extracellular Matrix
Yi-Pin Lin and John M. Leong
6. Analysis of Lipids and Lipid Rafts in Borrelia
Alvaro Toledo, Zhen Huang, Jorge Benach, and Erwin London
7. Trace Element AnalysisofBorrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry
Xin Li, Peng Wang, Anthony Lutton, and John Olesik
8. Identification and Characterization of Borrelia burgdorferiComplement-binding Proteins
Peter Kraiczy
9. Artificial Infection of Ticks with Borrelia burgdorferi using a Microinjection Method and their Detection in vivo using Quantitative PCR Targeting flaB RNA
Alexis A. Smith, Xiuli Yang, Erol Fikrig, and Utpal Pal
10. Borrelia burgdorferi Microarray Analysis
Radha Iyer and Ira Schwartz
11. Borrelia burgdorferi Transcriptome Analysis by RNA-sequencing
Lybecker Mand Henderson KC
12. Selection of Borrelia burgdorferi Promoter Sequences Active during Mammalian Infection Using In Vivo Expression Technology
Philip P. Adams and Mollie W. Jewett
13. Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins
J. Seshu , Trever C. Smith II, Ying-Han Lin, S.L. Rajasekhar Karna, Christine L. Miller, and Tricia Van Laar
14. Identification of Acetylated Proteins in Borrelia burgdorferi
Youyun Yang, Alan Wolfe, and X. Frank Yang
15. Genetic Transformation and Complementation
D. Scott Samuels, Dan Drecktrah, and Laura S. Hall
16. Genome-wide Mutagenesis in Borrelia burgdorferi
Tao Lin and Lihui Gao
17. Generation of Conditional Mutants in Borrelia burgdorferi
Jacob I. Latham and Jon S. Blevins
18. Detection of Bioluminescent Borrelia burgdorferi from in vitro
Jenny A. Hyde and Jon T. Skare
19. Analysis of Borrelia burgdorferi Proteome and Protein-protein Interactions
Xiuli Yang, Meghna Thakur, Juraj Koci, Alexis A. Smith, Preeti Singh, Xuran Zhuang, Kamoltip Promnares, Yan Wang, Ozlem Buyuktanir, and Utpal Pal
20. Two Photon Intravital Microscopy of Lyme Borrelia in Mice
Alexia A. Belperron, Jialing Mao, and Linda K. Bockenstedt
21. Investigating Human Dendritic Cell Immune Responses to Borrelia burgdorferi
Lauren M.K. Mason and Joppe W.R. Hovius
22. Phagocytosis Assays for Borrelia burgdorferi
Juan Anguita, Ana Carreras-González, and Nicolas Navasa
23. Measuring Borrelia burgdorferi Motility and Chemotaxis
Kai Zhang and Chunhao Li
24. In vitro Models of Cutaneous Inflammation
Quentin Bernard, Benoît Jaulhac, and Nathalie Boulanger
25. Magnetic Isolation of Phagosomes Containing Toll like Receptor Ligands
Tanja Petnicki-Ocwieja and Linden T. Hu
26. Xenodiagnosis using Ixodes scapularis Larval Ticks in Humans
Siu-Ping Turk, Carla Williams, and Adriana Marques
27. Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization
Maria Gomes-Solecki and Luciana Richer
This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi. Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention of B. burgdorferi infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Borrelia burgdorferi: Methods and Protocols aims to ensure successful results in the further study of this vital field of biomedical research.