Blood-Brain Barrier

ISBN-13: 9781493989454 / Angielski / Twarda / 2018 / 367 str.

Tatiana Barichello

Spis treści

Series Preface…

Preface…

Table of Contents…

Contributing Authors…

1. An Overview of the Blood-Brain Barrier

Tatiana Barichello, Allan Collodel, Rodrigo Hasbun, and Rodrigo Morales

2. Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics

Brain M. Kopec, Kavisha R. Ulapane, Mario E.G. Moral, and Teruna J. Siahaan

3. Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modelling of the Blood-Brain Barrier

Malgorzata Burek and Carola Y. Förster

4. In Vitro BBB Models: Working with Static Platforms and Microfluidic Systems

Mohammad A. Kaisar, Vinay V. Abhyankar, and Luca Cucullo

5. In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress in Brain Microvascular Endothelial Cell Phenotype

Keith D. Rochfort and Philip M. Cummins

6. Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain Barrier

Balaji Srinivasan and Aditya Reddy Kolli

7. Cell Penetrating Peptides as Theranostics against Impaired Blood-Brain barrier Permeability: Implications for Pathogenesis and Therapeutics Treatment of Neurodegenerative Disease

Swapna Bera and Anirban Bhunia

8. Microbial Translocation of the Blood-Brain Barrier

Charles T. Spencer and Mireya G. Ramos-Muniz

9. Transport across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay System

Sen Takeda and Keishi Narita

10. Drosophila as a Model to Study the Blood-Brain Barrier

Cameron Love and Brigitte Dauwalder

11. Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain Barrier

Tianzhi Yang and Shuhua Bai

12. Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents

Fabricia Petronilho, Julia L. Goldman, and Tatiana Barichello

13. Experimental Tools to Study the Regulation and Function of the Choroid Plexus

Isabel Gonçalves, Telma Quintela, Ana Catarina Duarte, Peter Hubbard, Graça Baltazar, Christian Schwerk, Andrea Carmine Belin, Joana Tomás, and Cecília Reis A. Santos

14. Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid acorss the Blood-Cerebrospinal Fluid Barrier

Shin-ichi Akanuma, Yoshiyuki Kubo, and Ken-ichi Hosoya

15. In Vivo Analysis to Study Transport across the Blood-Retinal Barrier

Yoshiyuki Kubo, Shin-ichi Akanuma, and Ken-ichi Hosoya

16. Increasing BBB Permeability via Focused Ultrasound: Current Methods in Pre-Clinical Research

Dallan McMahon, Charissa Poon, and Kullervo Hynynen

17. Evaluation of Blood-Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evan’s Blue Extravasation

Trish Domi, Faraz Honarvar, and Andrea Kassner

18. Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy

Aline Silva de Miranda, Thiago Macedo Cordeiro, Milene Alvarenga Rachid, and Antônio Lúcio Teixeira

19. Molecular Imaging of Blood-Brain Barrier Permeability in Preclinical Models Using PET and SPECT

Vijayasree V. Giridharan, Tatiana Barichello, and Sudhakar Selvaraj

20. Biomarkers for Microvascular Proteins Detection: Blood-Brain Barrier Injury and Damage Measurement

Pavani Sayana, Jean Pierre Oses, Tatiana Barichello, and Vijayasree V. Giridharan

Blurb

This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Cutting-edge and thorough, Blood-Brain Barrier is a valuable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches.