Biotechnology of Plant Secondary Metabolism: Methods and Protocols

ISBN-13: 9781493933914 / Angielski / Twarda / 2016 / 180 str.

Arthur Germano Fett-Neto

Spis treści

1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal
Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan

2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
Thanise Nogueira, Füller, Júlio C

ésar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corrêa, and Arthur G. Fett-Neto

3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines
Júlio César de Lima, Thanise Nogueira F

üller, Fernanda de Costa, Kelly C.S. Rodrigues-Corrêa, and Arthur G. Fett-Neto

4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier

5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello

6. Production of Recombinan

t Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli
Flávia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera

7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
Vishal Singh Negi and Dulal Borthakur

8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast
Anita Emmerstorfer and Harald Pichler

9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello

10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
Morten Egevang Jørgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier

11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
Márcia R. de Almeida and Martina V. Strömvik

12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
Inês Carqueijeiro, Viviana Martins, Henrique Noronha, Hernâni Gerós, and Mariana Sottomayor

13. Protoplast Transformation as a Plant-Transferable Transient Expression System
Patrícia Duarte, Diana Ribeiro, Inês Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor

14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System
Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang

15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs
Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang

Blurb

This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.