Biosynthesis and bioactivity of nano-ZrO2 using F. oxysporum extract

ISBN-13: 9786205516645 / Angielski / Miękka / 88 str.

Ghada Mohammed El-Sayed El-Shamy; Ashraf Abdelmontaleb Elsayed; Attia Ahmed Attia

Opis

The current book describes the biosynthesis of ZrO2-NPs using F. oxysporum extract and characterization of their formation employing UV-vis spectroscopy, Energy Dispersive Analysis of X-ray (EDX), Fourier-transform infrared spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Selected Area Diffraction Pattern, and Scanning Electron Microscopy (SEM). The cytotoxic assessment revealed moderate cytotoxic activities against HePG-2, MCF-7, PC3, HeP2, and HeLa tumor, and normal WI-38 cell lines. The antimicrobial activity observed against Gram-positive and Gram-negative bacterial species specified that ZrO2-NPs demonstrated impending inhibitory action against Gram-negative bacterial species. Also, polyacrylamide gel electrophoresis was applied for the analysis of proteins that participated in the biosynthesis of ZrO2-NPs.The anionic detergent is responsible for destroying the native protein chains into the polypeptide units and entirely swamps the unique charge of the proteins along with adapting to the overall negative charge of all analyzed protein molecules. Therefore, ZrO2-NPs could be recommended for relevant biomedical applications after further in vivo studies.

O autorze

Ghada El-Shamy was born in Algharbiah, Elmahalla Elkubra, Egypt (1986). She received her B.Sc. 2007 from the faculty of science, Ain Shams University, Egypt. She obtained a Diploma in Applied Microbiology from the faculty of science, Mansoura University, Egypt, and an M.Sc. in 2022 from the faculty of science, Benha University, Benha, Egypt.