Bioremediation Protocols

ISBN-13: 9780896034372 / Angielski / Twarda / 1997 / 339 str.

David Sheehan

Opis

The many thousands of human-made and other chemical compounds present in the environment offer a serious challenge to our btosphere. It is appropriate, therefore, that our response to these products of human kno- edge and ingenuity should draw on a body of mtenstve scientific endeavor that is no less impressive. Bioremediation offers the possibility of harnessing the diversity of the biosphere to degrade, remove, alter, or otherwise detoxify these various chemicals. It brings together scientists from a wide variety of disciplines and backgrounds, such as microbiology, molecular biology, a- lytical chemistry, and chemical and environmental engineering, among o- ers. These different fields, each with its own individual approach, have actively contributed to the development of bioremediation research tn recent years. The prmcipal objective of Bioremediation Protocols is to make the fruits of some of this research available in a different format to that of the textbook or journal article. It provides a selection of clearly written laboratory pro- cols presented as stepwise, easy-to-follow mstructions. In common with p- wous volumes in this and the companion Methods in Molecular Biology series, an extensive "Notes" section is provided with each chapter. This contains u- ful mformation (of a type often not normally included m a research paper) supplementmg the protocol. Reviews and case studies are also included to provide a deeper context to the methods chapters.

Recenzje

"...a practical addition to the series of books in Methods in Biotechnology....It will be a useful reference book to those involved with experimental protocols in the area of bioremediation. Given the extremely wide range of pollutants, as well as the techniques that are or could be used in the bioremediation field, this book does a very good job in presenting a variety of methods,...Finally, the book ends with a comprehensive listing of abbreviations and acronyms and a useful subject index. This is a well-presented and useful book for researchers in the bioremediation field."-Applied Biochemistry and Biotechnology

Spis treści

Part I. Overview. Uses of Bacteria in Bioremediation, Emer Colleran. Granular Sludge Consortia for Bioremediation, Nina Christiansen, Indra M. Mathrani, and Birgitte K. Ahring. Part II. Protocols. Screening of Bacterial Products for Their Crude Oil Biodegradation Effectiveness, Albert D. Venosa, John R. Haines, and B. Loye Eberhart. Measurement of Biosurfactant-Enhanced Solubilization and Biodegradation of Hydrocarbons, Raina M. Miller and Yimin Zhang. Immobilization of Bacteria in Macro- and Microparticles, David B. Knaebel, Keith E. Stormo, and Ronald L. Crawford. Immobilization and Evaluation of Bacterial Cells in Bioreactors, Samir S. Sofer. Immobilization of Yeast and Algal Cells for Bioremediation of Heavy Metals, John R. Duncan, Dean Brady, and Brendan Wilhelmi. Enumeration of Hydrocarbon-Degrading Bacteria, Laura E. Rice and Barbara B. Hemmingsen. Molecular Methods for the Detection of Methanotrophs, Ian R. McDonald, Andrew J. Holmes, Elizabeth M. Kenna, and J. Colin Murrell. Measurement of Mutagenic Activity in Contaminated Soils, Shanna L. Collie and Kevin C. Donnelly. Conjugation-Mediated Gene Transfer in Bacterial Strains to Be Used for Bioremediation, Max Mergeay and Dirk Springael. Analysis of Pentachlorophenol in Soils for Use in Bioremediation Protocols, Ruth McGrath and Ian Singleton. Generation of Species-Specific DNA Probes for the Lignin Peroxidase Genes of White Rot Fungi, Patrick J. Collins and Alan D. W. Dobson. Biodegradation of Nitroaromatics by Microbes, Juan L. Ramos, Asunciön Delgado, Estrella Duque, Maria-Dolores Fandila, Matilde Gil, Ali Haïdoue, Gloria Lucchesi, Carmen Michán, and Rafael Salto. The Determination of Trace Elements in Biological and Environmental Samples Using Atomic Absorption Spectroscopy, John O'Halloran, Andrew R. Walsh, and Patrick J. Fitzpatrick. Quantifying Organic and Inorganic Tin Compounds in Environmental Samples, Joseph J. Cooney. Protocol for Determining Bioavailability and Biodegradation Kinetics ofOrganic Soil Pollutants in Soil Systems to Enhance Bioremediation of Polluted Soil Sites, Henry H. Tabak, Rakesh Govind, Chunsheng Fu, and Chao Gao. Methods for Evaluation of PCB Dechlorination in Sediments, John F. Quensen III and James M. Tiedje. Evaluation of PCB Dechlorination in Sediments, John F. Quensen III and James M. Tiedje. Part III. Case Studies. Microbial Degradation of Alkenylbenzenes, Kevin E. O'Connor and Alan D. W. Dobson. Heavy Metal Bioremediation of Soil, Ludo Diels. Application of Bioavailability and Biokinetics Protocol to Phenol and Polycyclic Aromatic Hydrocarbon Contaminants in Soil and Development of Bioavailability and Biokinetic Models for Soil Systems, Henry H. Tabak, Rakesh Govind, Chunsheng Fu, and Chao Gao. Index.

Blurb

In Bioremediation Protocols leading researchers from around the world present their time-tested genetic, chemical, and analytical techniques for studying specific pollutants and their remediation. Their expert procedures range widely from cell immobilization and screening to microbiological and analytical chemistry methods that are applied to such environmental pollutants as hydrocarbons, PAHs, PCBs, TBT, and heavy metals. These pathbreaking contributors have also included illuminating reviews and case studies intended to expand the useful range of the methods. In addition, they discuss such major issues in bioremediation as the design and use of bioreactors, genetic manipulation, and the preparation and analysis of environmental samples.

Bioremediation Protocols brings together the most useful work of scientists from a wide variety of disciplines. Its techniques are essential to harnessing the diversity of biological means now available to degrade, remove, alter, or otherwise detoxify the many harmful chemical compounds found in our environment today.