Bioreactors in Stem Cell Biology: Methods and Protocols

ISBN-13: 9781493982134 / Angielski / Miękka / 2018 / 238 str.

Kursad Turksen

Spis treści

1. Multi-Compartmental Hollow-Fiber-Based Bioreactors for Dynamic Three-Dimensional Perfusion Culture

            Eva Schmelzer and Jörg C. Gerlach

 

2. A Bioreactor to Apply Multi-Modal Physical Stimuli to Cultured Cells

            Jan-Christoph Edelmann, Lizzie Jones, Remi Peyronnet, Liang Lu, Peter Kohl, and Ursula Ravens

 

3. Aggregate and Microcarrier Cultures of Human Pluripotent Stem Cells in Stirred Suspension Systems

            Preeti Ashok, Yongjia Fan, Mahboubeh R. Rostami, and Emmanuel S. Tzanakakis

 

4. Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors

            Walaa Almutawaa, Leili Rohani, and Derrick E. Rancourt

 

5. Uniformed Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Stem Cells in a Rotary Suspension Bioreactor

            Xiaohua Lei, Zhili Deng, and Enkui Duan

 

6. Expansion of Human Mesenchymal Stem Cells in a Microcarrier Bioreactor

            Ang-Chen Tsai and Teng Ma

 

7. Large-Scale Expansion and Differentiation of Mesenchymal Stem Cells in Microcarrier-Based Stirred Bioreactors

            Sébastien Sart and Spiros N. Agathos

 

8. Bioreactor Expansion of Skin-Derived Precursor Schwann Cells

            Tylor Walsh, Jeff Biernaskie, Rajiv Midha, and Michael S. Kallos

 

9. Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment

            Sadman Sakib, Camila Dores, Derrick Rancourt, and Ina Dobrinski

 

10. Generation of Neural Progenitor Spheres from Human Pluripotent Stem Cells in a Suspension Bioreactor

            Yuanwei Yan, Liqing Song, Ang-Chen Tsai, Teng Ma, and Yan Li

 

11. Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells

            Daniel Simão, Francisca Arez, Ana P. Terasso, Catarina Pinto, Marcos F.Q. Sousa, Catarina Brito, and Paula M. Alves

 

12. Scalable Expansion of Human Pluripotent Stem Cell-Derived Neural Progenitors in Stirred Suspension Bioreactor Under Xeno-Free Condition             Shiva Nemati, Saeed Abbasalizadeh, and Hossein Baharvand

 

13. A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies

            Jonas Christoffersson, Gunnar Bergström, Kristin Schwanke, Henning Kempf, Robert Zweigerdt, and Carl-Fredrik Mandenius

 

14. Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies

            Sasitorn Rungarunlert, Joao N. Ferreira, and Andras Dinnyes

 

15. Whole Heart Construct Cultivation Under 3D Mechanical Stimulation of the Left Ventricle

            Jörn Hülsmann, Hug Aubin, Alexander Wehrmann, Alexander Jenke, Artur Lichtenberg, and Payam Akhyari

 

16. Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading

            Daniel W. Youngstrom and Jennifer G. Barrett

 

17. Bioengineered Models of Solid Human Tumors for Cancer Research

            Alessandro Marturano-Kruik, Aranzazu Villasante, and Gordana Vunjak-Novakovic

 

18. Development of a Bladder Bioreactor for Tissue-Engineering in Urology

            Niall F. Davis and Anthony Callanan

 

19. Use of Microfluidic Technology to Monitor the Differentiation and Migration of Human ESC-Derived Neural Cells

            Jiwoo Bae, Nayeon Lee, Wankyu Choi, Suji Lee, Jung Jae Ko, Baek Soo Han, Sang Chul Lee, Noo Li Jeon, and Jihwan Song

Blurb

This meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs.  Notable advances are being made in these latter approaches, prompting this collection of a variety of representative protocols that facilitate important modifications and novel approaches to bioreactors in stem cell research, contributed by both established and new investigators in this area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Bioreactors in Stem Cell Biology: Methods and Protocols will serve as an ideal guide for scientists seeking to increase our understanding of stem cells and their potential to repair and regenerate tissues and organs.