Bioreactors in Stem Cell Biology: Methods and Protocols

ISBN-13: 9781071620205 / Angielski / Miękka / 2023 / 273 str.

Kursad Turksen

Opis

This new edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Bioreactors in Stem Cell Biology: Methods and Protocols, Second Edition will benefit both established investigators and newcomers to this dynamic area of study.

Spis treści

1. Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines

            Angélique Schmid, Emanuel Kreidl, Martin Bertschinger, and Patrick Vetsch

 

2. Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor

            Aysegul Inam, Ezgi Rojda Taymaz, Mehmet Emin Uslu, Baris Binay, and Irem Deniz

 

3. Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volumed Bioreactors for Cell Culturing

            Elif Gencturk, Senol Mutlu, and Kutlu O. Ulgen

 

4. Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors

            Marites T. Woon, Puspa R. Pandey, and Inbar Friedrich Ben-Nun

 

5. High Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors

            Xiaohua Lei, Chiyuan Ma, Yujing Cao, Yue Xiong, Jian V. Zhang, and Enkui Duan

 

6. Integrating Human Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture

            Valerie Ho, Gerine Tong, Alan Lam, Shaul Reuveny, and Steve Oh

 

7. Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor

            Misha Teale, Valentin Jossen, Dieter Eibl, and Regine Eibl

 

8. Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor

            Huanye Xu, Zhongxiao Cong, Yuanyuan Zhang, Wei Liu, Xiaojun Yan, and Yanan Du

 

9. Optimized Method to Improve Cell Activity in 3D Scaffolds under a Dual Real-Time Dynamic Bioreactor System

            Flavia Pedrini, Moema A. Hausen, and Eliana A.R. Duek

 

10. In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

            Ziming Chen, Peilin Chen, Rui Ruan, and Minghao Zheng

 

11. Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor

            Kamil Wierzchowski and Maciej Pilarek

 

12. Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor

            Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, and Tiziana A.L. Brevini

 

13. Bioreactor-Based De-Epithelialization of Long-Segment Tracheal Grafts

            Alba E. Marin-Araujo, Siba Haykal, and Golnaz Karoubi

 

14. Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask

            Anastasiia Artuyants, Vanessa Chang, Gabrielle Reshef, Cherie Blenkiron, Lawrence W. Chamley, Euphemia Leung, and Colin L. Hisey

 

15. Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors

            Xuegang Yuan, Xingchi Chen, Changchun Zeng, David G. Meckes Jr., and Yan Li

 

16. Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model

            Max Wacker, Jan Riedel, Priya Veluswamy, Maximilian Scherner, Jens Wippermann, Heike Walles, and Jörn Hülsmann

 

17. A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)

            Julian Kopp and Oliver Spadiut

 

18. Applying Stirred Perfusion to 3D Tissue Equivalents to Mimic the Dynamic In Vivo Microenvironment

            Henry W. Hoyle, Claire L. Mobbs, and Stefan A. Przyborski

 

19. Bioreactor-Based Adherent Cells Harvesting from Microcarriers with 3D Printed Inertial Microfluidics

            Lin Ding, Reza Moloudi, and Majid Ebrahimi Warkiani