Biopolymer Methods in Tissue Engineering

ISBN-13: 9781617372803 / Angielski / Miękka / 2010 / 257 str.

Anthony P. Hollander; Paul V. Hatton

Opis

There is an urgent need to develop new approaches to treat conditions as- ciated with the aging global population. The surgeon s approach to many of these problems could be described as having evolved through three stages: Removal: Traditionally, diseased or badly damaged tissues and structures might simply be removed. This was appropriate for limbs and non-essential organs, but could not be applied to structures that were critical to sustain life. An additional problem was the creation of disability or physical deformity that in turn could lead to further complications. Replacement: In an effort to treat wider clinical problems, or to overcome the limitations of amputation, surgeons turned to the use of implanted materials and medical devices that could replace the functions of biological structures. This field developed rapidly in the 1960s and 1970s, with heart valve and total joint replacement becoming common. The term biomaterial was used increasingly to describe the materials used in these operations, and the study of biomaterials became one of the first truly interdisciplinary research fields. Today, biomaterials are employed in many millions of clinical procedures each year and they have become the mainstay of a very successful industry."

Recenzje

From the reviews:

"This small, 280-page volume is useful to people who are interested in using various laboratory techniques to conduct tissue engineering research. ... Tips on troubleshooting and avoiding pitfalls which appear at the end of nearly all chapters will be welcomed by users of the book. The goal of this book is to 'serve as a basic laboratory manual allowing tissue engineering scientists not only to access a wide range of techniques in one place, but also to have them described using a standard format'." (Annales de Chimie Science des Materiaux, Vol. 29 (4), 2004)

Spis treści

Processing of Resorbable Poly-a-Hydroxy Acids for Use as Tissue-Engineering Scaffolds Minna Kellomäki and Pertti Törmälä Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing Raphael Gorodetsky, Akiva Vexler, Lilia Levdansky, and Gerard Marx Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering Carlo Soranzo, Davide Renier, and Alessandra Pavesio Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering Steven H. Elder, Dana L. Nettles, and Joel D. Bumgardner Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2 Hyun D. Kim, John M. Wozney, and Rebecca H. Li Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications Manuela E. Gomes, Patrícia B. Malafaya, and Rui L. Reis Alginates in Tissue Engineering Marcy Wong Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering Yang Cao, Tristan I. Croll, Justin J. Cooper-White, Andrea J. O'Connor, and Geoffrey W. Stevens Modification of Materials With Bioactive Peptides Jennifer L. West Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering António J. Salgado, Manuela E. Gomes, Olga P. Coutinho, and Rui L. Reis Cell Seeding of Polymer Scaffolds Gordana Vunjak-Novakovic and Milica Radisic Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds Aileen Crawford and Sally C. Dickinson Bioreactor Culture Techniques for Cartilage-Tissue Engineering David A. Lee and Ivan Martin Microscopic Methods for the Analysis of Engineered Tissues Sally Roberts and Janis Menage Transmission Electron Microscopy ofTissue-Polymer Constructs Paul V. Hatton Application of Microscopic Methods for the Detection of Cell Attachment to Polymers John Hunt and Deborah Heggarty Biochemical Methods for the Analysis of Tissue-Engineered Cartilage Wa'el Kafienah and Trevor J. Sims Real-Time Quantitative RT-PCR Assays Ivan Martin and Oliver Frank Mechanical Testing of Cell-Material Constructs: A Review John Kisiday, Alex Kerin, and Alan Grodzinsky Index

Blurb

Because the rapidly developing field of tissue engineering encompasses all the major scientific disciplines-including materials science, polymer chemistry, biology, and medicine-it has often been difficult to uncover the full range of laboratory methods required in tissue engineering. In Biopolymer Methods in Tissue Engineering, expert laboratory researchers bring together in a standard format all the diverse laboratory methods needed to perform state-of-the-art tissue engineering research. Topics range from the synthesis, processing, and characterization of specific biomaterials, through the successful use of scaffolds in the engineering of tissues, to techniques useful in evaluating the biological quality of scaffold-engineered tissues. Subjects of special interest include the incorporation of biological molecules into scaffold biomaterials and the use of a wide range of methods and techniques to generate a comprehensive description of cell-polymer construct quality. Additional techniques can be used to develop effective in vitro models for drug evaluation or to investigate cell and tissue differentiation. Each readily reproducible protocol follows a uniform format that includes essential background information, step-by-step instructions by an expert, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Biopolymer Methods in Tissue Engineering offers both advanced and novice investigators a diverse collection of readily reproducible techniques for generating living cell-polymer constructs in their laboratories and for evaluating their biological quality.