Biomolecular Interactions Part a: Volume 166

ISBN-13: 9780128233511 / Angielski / Twarda / 2021 / 368 str.

Arun K. Shukla

Spis treści

1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET

2: Evaluating functional ligand-GPCR interactions in cell-based assays

3: Assays for detecting arrestin interaction with GPCRs

4: BRET-based assay to specifically monitor ß2AR/GRK2 interaction and ß-arrestin2 conformational change upon ßAR stimulation

5: Cannabinoid Receptor CB1 and CB2 Interacting Proteins: Techniques, Progress and Perspectives

6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization

7: Nanobodies as Sensors of GPCR Activation and Signaling

8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells

9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology

10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells

11: Methods for Binding Analysis of Small GTP-Binding Proteins with their Effectors

12: Protein-protein interactions in the brain: protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho- proteomics analysis

13: Protein-Protein Interactions at a glance: protocols for the visualization of biomolecular interactions

14: Interactions between Noncoding RNAs as Epigenetic Regulatory Mechanisms in Cardiovascular Diseases