Biomedical Epr, Part A: Free Radicals, Metals, Medicine, and Physiology. Part B: Methodology, Instrumentation, and Dynamics

ISBN-13: 9780387231273 / Angielski / Twarda / 2008 / 992 str.

Sandra S. Eaton; Gareth R. Eaton; Lawrence J. Berliner

Opis

Biomedical EPR - Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70^th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features:
-Free Radicals in Medicine
-Radicals in vivo and in Model Systems, and their Study by Spin Trapping
-In vivo EPR, including Oximetry and Imaging
-Time Domain EPR at Radio Frequencies
-EPR of Copper Complexes: Motion and Frequency Dependence
-Time Domain EPR and Electron Spin Echo Envelope Modulation. Biomedical EPR - Part B focuses on applications of EPR techniques and instrumentation, with applications to dynamics. The book celebrates the 70^th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field that lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features:
-EPR Techniques including Saturation Recovery, ENDOR, ELDOR, and Saturation Transfer
-Instrumentation Innovations including Loop Gap Resonators, Rapid Mixing, and Time Locked Sub-Sampling
-Motion in Biological Membranes
-Applications to Structure Determination in Proteins
-Discussion of Trends in EPR Technology and Prognosis for the Future.

Spis treści

Part A: Introduction.- An Appreciation of Jim Hyde and the National Biomedical ESR Center.- Free Radicals and Medicine.- Superoxide Formation from Nitric Oxide Synthase: A Spin-Trapping Approach.- In Vivo Spin Trapping.- Post-Processing Strategies in EPR Spin-Trapping.- Melanin.- Applications of Spin Labels to Membrane Bioenergetic (Photosynthethic Systems of Higher Plants).- EPR Spectroscopy of Function In Vivo.- EPR Oximetry in Biological and Model Samples.- In Vivo EPR Imaging.- Time Domain Radio Frequency EPR Imaging.- Copper Biomolecules in Solution.- Low Frequency EPR of Cu²⁺ in Proteins.- ESEEM of Biological Systems.- Index.

Part B: Saturation Recovery.- Loop Gap Resonators.- EPR Interfaced to Rapid Mixing.- Applications of Angle Selected Electrons Nuclear Double Resonance to Characterize Structure Solvent in Small Molecules and Macromolecules.- Solution-ENDOR of Some biologically Interesting Radical Ions.- Electron-Electron Double Resonance.- Digital Detection in EPR.- Distance Measurement by Pulse EPR-ESR and Molecular Dynamics.- Site-Directed Spin Labeling.- Saturation Transfer Studies of Biological Membranes.- Saturation Transfer EPR: Rotational Dynamics of Membrane Proteins.- Trends in EPR Technology.- Prognosis.- Index.

O autorze

Prof. Gareth R. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. His research interests include EPR instrumentation, distance measurements in proteins, EPR of metal ions in biological systems, and electron spin relaxation times. Prof. Sandra S. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. Her research interests include distance measurements in proteins, EPR of metal ions in biological systems, electron spin relaxation times, and EPR instrumentation. The Eatons co-organize an annual EPR Symposium in Denver. Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR).