Bioluminescent Imaging: Methods and Protocols

ISBN-13: 9781493960675 / Angielski / Miękka / 2016 / 276 str.

Christian E. Badr

Opis

Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.

Recenzje

From the reviews:

"The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields." (Omer Iqbal, Doody's Book Reviews, January, 2014)

Spis treści

1. Bioluminescence Imaging: Basics and Practical Limitations

            Christian E. Badr

 Part I: In Vitro Bioassays

 2. Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells

            Junji Chida and Hiroshi Kido

 3. Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter

            Ludovic Tricoire and Bertrand Lambolez

 4. Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture

            M. Hannah Degeling,  M. Sarah S. Bovenberg, Marie Tannous, and Bakhos A. Tannous

 Part II: Imaging Molecular and Cellular Events

 5. Split Gaussia Luciferase for Imaging Ligand-Receptor Binding

            Kathryn E. Luker and Gary D. Luker

 6. Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell

            Takahiro Suzuki and Satoshi Inouye

 7. Bioluminescence Reporter Gene-Based Detection of MicroRNAs

            Hae Young Ko, Young Sik Lee, and Soonhag Kim

 8. Monitoring of Transcriptional Dynamics of HIF and NFκB Activities

            Miguel A.S. Cavadas and Alex Cheong

 9. Real-Time Bioluminescent Tracking of Cellular Population Dynamics

            Dan Close, Tingting Xu, Steven Ripp, and Gary Sayler

 10. Fabrication of Bioluminescent Capsules and Live-Cell Imaging

            Sung Bae Kim

 Part III: Imaging of Disease (Pathogenesis)

 11. The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice

            Scott J. Goldman and Shengkan Jin

 12. Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis

            Hiroshi Yamashita, Dan T. Nguyen, and Euiheon Chung

 13. Bioluminescence Imaging of Fungal Biofilm Development in Live Animals

            Greetje Vande Velde, Sona Kucharikova, Patrick Van Dijck, and Uwe Himmelreich

 14. Bioluminescent Imaging of Bacteria During Mouse Infection

            Jonathan M. Warawa and Matthew B. Lawrenz

 Part IV: Therapeutic Imaging

 15. Cell-Based Bioluminescence Screening Assays

            Romain J. Amante and Christian E. Badr

 16. Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice

            Casey A. Maguire

17. Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase

            Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi

 Part V: Emerging Technologies

18. Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay

            Sjoerd van Rijn, Thomas Würdinger, and Jonas Nilsson

19. Non-Invasive In Vivo Monitoring of Extracellular Vesicles

            Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield

20. In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL)

            Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Régis Tournebize, and Spencer Shorte