Biological Microarrays: Methods and Protocols

ISBN-13: 9781934115954 / Angielski / Twarda / 2010 / 324 str.

Ali Khademhosseini; Kahp-Yang Suh; Mohammed Zourob

Opis

Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods. Chapters address both the application of biological microarrays, including DNA/RNA, apatmer, proteins, tissues, oligonucleotides, carbohydrates, biomaterials, cells, bacteria, and virus microarrays, and also explore the different techniques used for generating microarray platforms. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and revolutionary, Biological Microarrays: Methods and Protocols serves as a primary source for academics, practitioners, and professionals in related fields, including biologists, biotechnologists, biochemists, analytical chemists, and biomedical, physical, and microsystems engineers, to name a few, appealing to all of those interested in the present and future state of biological microarray research.

Spis treści

Biological Microarrays: Methods and Protocols Editors: Ali Khademhosseini, Suh Kahp-Yang, Mohammed Zourob Table of Contents Preface Contributors Part 1: Application of Biological Microarray 1. RNA and DNA Microarrays Tearina T. Chu and Stuart C. Sealfon 2. Aptamer Arrays Eva Baldrich 3. Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants Dmitriy V. Volokhov, Hyesuk Kong, Keith Herold, Vladimir E. Chizhikov, and Avraham Rasooly 4. Protein MicroArrays printed from DNA MicroArrays Oda Stoevesandt, Mingyue He and Michael J Taussig 5. Lithographically-defined two- and three-dimensional tissue microarrays Esther W. Gomez and Celeste M. Nelson 6. Ratiometric lectin microarray analysis of the mammalian cell surface glycome Ku-Lung Hsu, Kanoelani Pilobello, Lakshmipriya Krishnamoorthy, and Lara K. Mahal 7. Cell microarrays based on hydrogel microstructures for the application to cell-based biosensor Won-Gun Koh 8. Fabrication of Bacteria and Virus Microarrays Based on Polymeric Capillary Force Lithography Pil J. Yoo 9. 3D Polymer Scaffold Arrays Carl G. Simon, Jr., Yanyin Yang, Shauna M. Dorsey, Murugan Ramalingam, Kaushik Chatterjee Part 2: Methods for Microarray Generation 10. PDMS microfluidic capillary systems for patterning proteins on surfaces and performing miniaturized immunoassays Mateu Pla-Roca and David Juncker 11. Merging Photolithography and Robotic Protein Printing to Create Cellular Microarrays Ji Youn Lee and Alexander Revzin 12. Generation of Protein and Cell Microarrays on Functionalized Surfaces Yoo Seong Choi and Chang-Soo Lee 13. Microprinting of Liver Micro-organ for Drug Metabolism Study Robert C. Chang, Kamal Emami, Antony Jeevarajan, Honglu Wu, Wei Sun 14. Microcontact printing Yunyan Xie and Xingyu Jiang 15. Micromolding for the Fabrication of Biological Microarrays Ashley L. Galloway, Andrew Murphy, Jason P. Rolland, Kevin P. Herlihy, Robby A. Petros, Mary E. Napier, and Joseph M. DeSimone 16. Progress Report on Microstructured Surfaces Based on Chemical Vapor Deposition Yaseen Elkasabi and Joerg Lahann 17. Methods for Forming Human Microvascular Tubes in Vitro and Measuring Their Macromolecular Permeability Gavrielle M. Price and Joe Tien 18. Microarray Bioinformatics Robert P. Loewe and Peter J. Nelson