Biological Aging: Methods and Protocols

ISBN-13: 9781493963133 / Angielski / Miękka / 2017 / 354 str.

Trygve O. Tollefsbol

Opis

This book details recent breakthroughs in technology that have served to advance the study of aging. It features step-by-step, readily reproducible laboratory protocols as well as key tips on troubleshooting and avoiding known pitfalls.

Spis treści

1. Cell Senescence Culturing Methods

Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol

 

2. Digital Image Analysis of Cells Stained with the Senescence-Associated Beta-Galactosidase Assay

Liran I. Shlush and Sara Selig

 

3. Analysis of Biomarkers of Caloric Restriction in Aging Cells

Yuanyuan Li and Trygve O. Tollefsbol

 

4. Cell Sorting of Young and Senescent Cells

Graeme Hewitt, Thomas von Zglinicki, and João F. Passos

 

5. Studying the Replicative Life Span of Yeast Cells

David A. Sinclair

 

6. Methods for Creating Mutations in C. elegans that Extend Lifespan

Dayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong

 

7. Aging Studies in Drosophila Melanogaster

Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou

 

8. The Use of Calorie Restriction Mimetics to Study Aging

Munehiro Kitada and Daisuke Koya

 

9. Using Somatic-Cell Nuclear Transfer to Study Aging

Satoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama

 

10. Induction of Cellular Senescence by Oncogenic RAS

Romi Gupta and Narendra Wajapeyee

 

11. Methods of Cellular Senescence Induction Using Oxidative Stress

Zhe Wang, Dandan Wie, and Hengyi Xiao

 

12. Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice

 Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski

13. Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors

Dejia Li and Dongsheng Duan

 

14. Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration

Nadav Kimelman Bleich, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli , Zulma Gazit , Gadi Pelled, and Dan Gazit

15. Exometabolomic Mapping of Caenorhabditis elegans – A Tool to Non-Invasively Investigate Aging

Robert J. Mishur, Jeffrey A. Butler, and Shane L. Rea

 

16. Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing

Gilberto Vargas-Alarcón and Carmina Flores-Domínguez

 

17. Gel Electrophoresis-based Proteomics of Senescent Tissues

Steven Carberry and Kay Ohlendieck

 

18.  Single-Cell Semiconductor Sequencing

Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz

 

19. Application of DNA Microarray Technology to Gerontological Studies

Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan

 20. Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells

Carmen M. Koch and Wolfgang Wagner

 

21. Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging

Leonid L. Moroz  and Andrea B. Kohn

 

Blurb

The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Biological Aging:  Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.