1. Monospecies and polymicrobial biofilms in static and flow environment Mohd Hafiz Arzmi 2. Methods used to study biofilms 3. Spatial analysis of bacterial biofilms Romain Briandet, Virgile Guéneau, Raphael Charron, Vlad Costache and Arnaud Bridier 4. Biofilm grown in bioreactors Edvaldo Antonio Ribeiro Rosa 5. Single-cell analysis of subpopulations within biofilms using microscopy, flow cytometry, and imaging flow cytometry Ilana Kolodkin-Gal 6. Microscopy analysis of biofilm-mineral interactions or mineral formation within biofilms Karim Benzerara 7. Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions Dmitri Mavrodi 8. Studying Gene Expression in Biofilms Boo Shan Tseng