Biochemistry of Vitamin B6: Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, Held in Turk

ISBN-13: 9783034899895 / Angielski / Miękka / 2011 / 469 str.

P. Christen;Korpela

Opis

The ecent Vitamin Be-Meeting in Tu ku, FinLand, has continued a Long-standing t1'O.dition; it ws the seventh intemationaL congNss devoted to the "ChemicaL and BioLogicaL Aspects of Vitamin Be-Cata- Lysis." The fi st meeting ws heLd 1962 in Rome foUo1JJed by the meetings in Moscow (1966), Nagoya (1967), Lening1'O.d (1974), To onto (1979), and Athens (1983). The p oceedings of a meeting p ovide a "snapshot" of a pa ticuLa fieLd of science. Not unLike the photog phic p oduct they affo d a topicaL eco of the goings-on, at pe haps the expense of some inforrmation on detaUs. These Proceedings focus on the st uctu L and mechanistic aspects of vitamin B6-dependent enaymes; h01JJeve, othe topics discussed nge f om nonenaymic eactions of vitamin B6 compounds to nut tionaL, medicaL, and even appLied indust aL as- pects. The Opening Session of the Meeting ws dedicated to the memo y of the Late ALe: x: ande E. B unstein, who fifty yea s ago discove ed t nsamination as a metaboUc eaction. The contT'ibutions honoT'ing P ofesso B unstein and his Ufewo k a e coUected in the fi st section of the oceedings.

Spis treści

Prologue in Honour of Alexander E. Braunstein 1902–1986.- The Discovery of Enzymatic Transamination and Its Biological Significance.- Professor Alexander E. Braunstein; Some Reminiscences.- Closing Remarks.- Molecular Biology and Site-Directed Mutagenesis.- Genes, Biosynthesis, and Intracellular Processing of the Isoenzymes of Aspartate Aminotransferase.- Structure-Function Relation of the Presequence of a Precursor to Pig Mitochondrial Aspartate Aminotransferase.- Molecular Cloning and in vivo Expression of the Precursor to Rat Mitochondrial Aspartate Aminotransferase.- Rat Cytosolic Aspartate Aminotransferase; Molecular Cloning of cDNA and Expression in E. coli.- D-Amino Acid Aminotransferase from a Thermophile, Bacillus sp. YM-1: Enzymological Properties, Cloning of the Gene, and the Amino Acid Sequence.- The Subunit Structure of Porcine Ornithine Aminotransferase and Mechanism of Proteolytic Modification of Its Putative Precursors in Mitochondria.- Hepatic Systems Degrade Tyrosine Aminotransferase.- Demonstration of Immunochemical Cross-Reactivity of Dopa Decarboxylase and Histidine Decarboxylase Using Antibodies against the Two Enzymes.- Mechanistic Analysis of the Aspartate Aminotransferase Active Site Mutants — Y70F, K258A, and R292D.- Mutation of Lysine 258 to Arginine in the Active Site of Aspartate Aminotransferase.- Bacterial Tryptophan Synthetase: A Multienzyme Complex Which Contains Pyridoxal Phosphate.- Site-specific Mutations in E. coli Maltodextrin Phosphorylase with Differential Effects on Substrate Binding and Catalysis.- Aminotransferases.- Spatial Aspects of Catalysis by Mitochondrial Aspartate Aminotransferase.- Recent Studies on Mitochondrial Aspartate Aminotransferase: Structure and Mechanism.- X-Ray Crystallographic, 19F-NMR and UV-Visible Spectroscopic Studies of Pig Heart Cytosolic Aspartate Aminotransferase.- Studies of the Inactivated Coenzyme in the Beta Sub form of Cytosolic Aspartate Aminotransferase.- Studies on the Mechanism of Action of Aspartate Aminotransferase by Cryoenzymological and Isotope Techniques.- Aspartate Aminotransferase from the Thermoacidophile Archaebacterium Sulfolobus solfataricus.- Coenzyme Reorientations in the Active Sites of Aspartate Aminotransferase Isoenzymes.- Phosphorus-31 Nuclear Magnetic Resonance Studies on Apoaspartate Aminotransferase Reconstituted with Cofactor Analogues.- Fourier Transform Infrared Spectroscopic Analysis of Phosphate Bound to the Aspartate Aminotransferase Isoenzymes.- Carboxylate Inhibition of Apoaspartate Aminotransferase.- The Reversible Oxidation of Vicinal SH Groups in 4-Aminobutyrate Aminotransferase. Probes of Conformational Changes.- Branched-Chain Amino Acid Aminotransferase from Escherichia coli.- Crystallographic and Solution Studies on Phosphoserine Aminotransferase (PSAT) from E. coli.- Studies on Liver GABA-Transaminase.- A Novel Aminotransferase Concerned with the Metabolism of Dimethylarginines in Rats.- Other Vitamin B6-Enzymes of Amino Acid Metabolism.- Chemical Probes of Substrate Channeling in the E. coli Tryptophan Synthase ?2?2 Bienzyme Complex.- Phosphorus-31 NMR Study of Pyridoxal 5?-Phosphate Binding to Escherichia coli Tryptophan Synthase Modified by Limited Proteolysis.- Glutamate Decarboxylase: Preliminary Crystallographic Data.- Analysis of the Glutamate Decarboxylase Reaction by Membrane Inlet Mass Spectrometry.- Proteolytic Cleavage of Pig Kidney Dopa Decarboxylase.- The Reaction of Dopa Decarboxylase with 5?-Hydroxytryptophan Isomers.- Sequence Studies on Vitamin B6-Dependent Enzymes: Serine Hydroxymethyltransferase and Glutamate Decarboxylase.- Stereochemistry of the Threonine Cleavage Complex.- The Substrate Specificity of Tyrosine Phenol-Lyase During the Different Stages of Enzymatic Process.- Structural and Catalytic Properties of Citrobacter intermedius Tyrosine Phenol-Lyase.- L-Methionine Y—Lyase: Its Essential Cysteine Residues.- Diaminopropionate Ammonia-Lyase of Salmonella typhimurium.- Interactions of Tryptophanase with Substrate Analogues.- S-Carboxymethylcysteine Synthase from E. coli.- Copper-Containing Amine Oxidases (EC 1.4.3.6) Have Covalently-Bound PQQ and not PLP as Organic Cofactor.- Phosphorylases.- The Role of the Pyridoxal Phosphate in Glycogen Phosphorylase b.- Phosphorylase Isozymes of Higher Plants.- The Polarized Single Crystal Electronic Absorption Spectrum of Phosphorylase b.- Crystallization and Structure Determination of Potato Phosphorylase at 3 Å Resolution.- Quaternary Structure and Regulatory Properties of Phosphorylase Kinase from Avian Skeletal Muscle.- Purification and Properties of Smooth Muscle Phosphorylase from Bovine Stomach.- Thermodynamic Study of the Binding of AMP to Glycogen Phosphorylase a and b.- Enzyme-Activated and Related Irreversible Inhibitors.- Enzyme-Activated Inhibitors of Bacterial D-Amino Acid Transaminase as Antimicrobial Agents.- Selective Inhibition of PLP-Dependent Enzymes by Hydroxylamine Derivatives.- On the Mechanism of Ornithine Decarboxylase Inhibition by 3-Aminooxypropylamine.- Kinetic Studies on Binding of Mitochondrial and Cytosolic Aspartate Aminotransferases with Suicide Substrate, Gostatin.- Inactivation of Bacterial ?-Amino Acid: Pyruvate Transaminase with Se-Hypotaurine.- Absence of Stereospecificity in the Suicide Inhibition of Ornithine Decarboxylase.- Enzyme-Activated Irreversible Inhibition of Bacterial and Plant Arginine Decarboxylase, by either Substrate or Product Analogues.- Cystathionine ?-Synthase and ?-Cystathionase have Conmon Peptide Sequences of the Active Sites and Are Inhibited Similarly by Aminoethoxyvinylglycine.- Non-Enzymic Reactions of Vitamin B6.- Micellar Catalysis of Pyridoxal-Dependent Reactions.- A New Approach to the Study of Pyridoxal 5?-Phosphate Schiff Bases.- Enthalpy, Entropy and Gibbs Energy Changes Associated with the Ionization of Vitamin B6 Molecules in Water-Dioxane Systems.- NMR Detection of a Delocalized Carbanion Intermediate in the Transamination Reactions of the Metal Chelates of Schiff Bases of Vitamin B6.- Copper(II) Chelates of an Intermediate Species Absorbing in the 500-nm Region.- Co-Ordination Compounds of Pyridoxol with Chromium and Manganese.- A Novel Method to Study the Topography of Transmembrane Proteins Using Pyridoxal 5?-Phosphate.- Metabolic, Nutritional, and Medical Aspects.- Glutamine and Asparagine Transaminases.- Rat Aspartate Aminotransferase: Regulation of Its mRNA and Its Contribution to Gluconeogenesis.- Vitamin B6 Deficiency Enhances End-Organ Sensitivity to Steroid Hormones.- Partial Purification and Characterization of Vitamin B6-P Phosphatases from Human Erythrocytes.- Pyridoxine Uptake by Proximal Tubular Epithelial Cells Isolated from Rat Kidney.- Influence of Vitamin B6 Nutritional Status upon Plasma Kinetics of PLP after I. V. Dosing to Rats.- Vitamin B6- Status of Finnish and Dutch Elderly.- Metabolism of Vitamin B6 by Human Subjects with and without Normal Liver Function.- Vitamin B6 Supplementation in Juvenile Neuronal Ceroid Lipofuscinosis Patients.- Bacterial Methionine -Lyase as a Possible Antitumor Agent.- A New Approach to the Pathogenetic Treatment of Psoriasis Using Coenzyme Inhibitors.- Biotechnology.- Enantiomer Synthesis of Amino Acids with Pyridoxal Enzymes.- Vitamin B6-Dependent Reactions in Organic Solvents.- ?—Amino—?— Caprolactam Racemase for L-Lysine Production.- Enzymatic Production of L-Cysteine from DL-2-Aminothiazoline- 4-Carboxylic Acid.- Industrial Production of L-Tryptophan from Indole and DL-Serine with two PLP-Dependent Enzymes.