Basophils and Mast Cells: Methods and Protocols

ISBN-13: 9781493954094 / Angielski / Miękka / 2016 / 251 str.

Bernhard F. Gibbs; Franco H. Falcone

Opis

In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most.Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.

Spis treści

Part I: Introduction & Historical Background

 

1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease

            Ana Olivera and Juan Rivera

 

Part II: Obtaining the Cells: Purification, Culture, Counting, and Cell Lines

 

2. Purification of Basophils from Peripheral Human Blood

            Franco H. Falcone and Bernhard F. Gibbs

 

3. Mast Cell Purification Protocols

            Jasmine Farrington, Elizabeth P. Seward, and Peter T. Peachell

 

4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells

            Hans Jürgen Hoffmann

 

5. Generation of Mast Cells from Murine Stem Cell Progenitors

            Emily J. Swindle

 

6. Integration of the Human Dermal Mast Cell into the Organotypic Coculture Skin Model

            Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc

 

7. The Absolute Basophil Count

            Elena Borzova and Clemens A. Dahinden

 

8.  Detection of Mast Cells and Basophils by Immunohistochemistry

            Andrew F. Walls and Cornelia Amalinei

 

9. Mast Cell and Basophil Cell Lines: A Compendium

            Egle Passante

 

Part III: Whole Cells: Identification in Tissues, Signaling Pathways, Gene Silencing, and Reporters

 

10. Measuring Histamine and Cytokine Release from Basophils and Mast Cells

            Bettina M. Jensen, Sidsel Falkencrone, and Per S. Skov

 

11. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques

            Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo

 

12. Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis

            Monika Bambousková, Zuzana Hájková, Pavel Dráber, and Petr Dráber

 

13. Use of Humanized Rat Basophil Leukaemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum

            Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone

 

14. Gene Silencing Approaches in Mast Cells and Primary Human Basophils

            Vadim V. Sumbayev and Bernhard F. Gibbs

 

15. Basophil Stimulation and Signaling Pathways

            Edward F. Knol and Bernhard F. Gibbs

 

16. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells

            José Mário Morgado, Laura Sánchez-Muñoz, Cristina Teodósio, and Luís Escribano

 

Part IV: Whole Organism: Disease and Knockout Models

 

17. Identification of Murine Basophils by Flow Cytometry and Histology

            Christian Schwartz and David Voehringer

 

18. Mast Cell Mediated Reactions In Vivo

            Vladimir Andrey Giménez-Rivera, Martin Metz, and Frank Siebenhaar