Basic Science Methods for Clinical Researchers

ISBN-13: 9780128030776 / Angielski / Miękka / 2017 / 382 str.

Morteza Jalali; Francesca Yvonne Louise Saldanha; Mehdi Jalali

Opis

Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science.

• Serves as a helpful guide for clinical researchers who lack a conventional science background
• Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms
• Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data
• Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Recenzje

"The information provided would be an excellent tool for a clinician in training.the content is best suited for those clinicians who lack basic science training or a research background."--Annals of Allergy, Asthma & Immunology

Spis treści

1. The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR 2. Methods of Cloning 3. Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos 4. Microarrays: An Introduction and Guide to Their Use 5. Analysis of Human Genetic Variations Using DNA Sequencing 6. Western Blot 7. The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research 8. Immunofluorescence 9. Cell Culture: Growing Cells as Model Systems In Vitro 10. Flow Cytometry 11. Transfection 12. In Vivo Animal Modeling: Drosophila 13. Zebrafish as a Research Organism: Danio rerio in Biomedical Research 14. Xenopus as a Model Organism for Biomedical Research 15. Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research

O autorze

Morteza Jalali is a British doctor-preneur who is fascinated by transformational healthcare innovation through synergies between clinicians, patients, industry and scientists. Born in Manchester, United Kingdom, at the age of 18 he commenced a medical degree at Manchester Medical School and undertook dedicated research training at The Healing Foundation Centre, University of Manchester, following the award of a Royal College of Surgeons student research scholarship. After the completion of his Bachelor's, Master's and medical degrees, Morteza decided to undertake a Doctor of Philosophy degree in stem cell biology at the Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute and was awarded a Cambridge Cancer Centre clinical research training fellowship to study at Emmanuel College, University of Cambridge. Morteza is committing to a career as a surgeon scientist by undertaking a period of postdoctoral research in the United States. He is currently a surgeon in training (plastic surgery theme) and honorary research fellow at the University of Oxford where he enjoys fulfilling a variety of clinical teaching commitments, having been a former faculty member at the University of Bristol. Morteza enjoys rowing, amateur boxing, graphic design, and spending time with family and friends in the United Kingdom and abroad. Clinical Research Fellow (M.Phil.), Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, USA Academic Foundation Doctor, Royal Liverpool University Hospital, Liverpool, UK