Barley: Methods and Protocols

ISBN-13: 9781493989423 / Angielski / Twarda / 2018 / 313 str.

Wendy A. Harwood

Spis treści

1. An Introduction to Barley: The Crop and the Model

            Wendy A. Harwood

 

2. Mutation Breeding in Barley:  Historical Overview

            Ludmila Ohnoutkova

 

3. A Practical Guide to Barley Crossing

            William T.B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, and Jerome D. Franckowiak

 

4. Barley Anther Culture

            Ludmila Ohnoutkova, Tomas Vlcko, and Mentewab Ayalew

 

5. Isolated Microspore Culture in Barley

            Patricio Esteves and François J. Belzile

 

6. TILLING in Barley

            Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, and Nils Stein

 

7. Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings

            Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, and Fiona M. Doohan

 

8. Agrobacterium-Mediated Transformation of Barley Immature Embryos

            Alison Hinchliffe and Wendy A. Harwood

 

9. Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses during Early Development

            Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, Michał Słota, Dorota Swiergolik, and Iwona Szarejko

 

10. Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy

            Marek Marzec and Michael Melzer

 

11. Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

            Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, and Isabelle Colas

 

12. Microarrays for High-Throughput Gene Expression Analysis of Barley

            Jenny A. Morris and Pete E. Hedley

 

13. Genome Engineering Using TALENs

            Goetz Hensel and Jochen Kumlehn

 

14. Creating Targeted Gene Knock-Outs in Barley Using CRISPR/Cas9

            Tom Lawrenson and Wendy Harwood

 

15. Genotyping-by-Sequencing on the Ion Torrent Platform in Barley

            Amina Abed, Gaétan Légaré, Sonia Pomerleau, Jérôme St-Cyr, Brian Boyle, and François Belzile

 

16. DNA Methylation Analysis in Barley and Other Species with Large Genomes

            Karolina Chwialkowska, Urszula Korotko, and Miroslaw Kwasniewski

 

17. High Resolution RT-PCR Analysis of Alternative Barley Transcripts

            Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P.G. Calixto, Linda Milne, Pete E. Hedley, Clare Booth, Robbie Waugh, and John W.S. Brown

 

18. Exome Capture for Variant Discovery and Analysis in Barley

            Micha Bayer, Jenny Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell, Robbie Waugh, and Pete E. Hedley

Blurb

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.