Baculovirus and Insect Cell Expression Protocols

ISBN-13: 9781588295378 / Angielski / Twarda / 2007 / 470 str.

David W. Murhammer

Opis

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins."

Spis treści

Table of Contents PART I. INTRODUCTION Chapter 1 Useful Tips, Widely Used Techniques and Quantifying Cell Metabolic Behavior David W. Murhammer PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES Chapter 2 Introduction to Baculovirus Molecular Biology Barbara J. Kelly, Linda A. King, and Robert D. Possee Chapter 3 Baculovirus Transfer Vectors Robert D. Possee and Linda A. King Chapter 4 Recombinant Baculovirus Isolation Linda A. King, Richard Hitchman, and Robert D. Possee Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System James A. Fornwald, Quinn Lu, Dayuan Wang, and Robert S. Ames PART III. INSECT CELL CULTURE Chapter 6 Available Lepidopteran Insect Cell Lines Dwight E. Lynn Chapter 7 Lepidopteran Insect Cell Line Isolation From Insect Tissue Dwight E. Lynn Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines Sprios N. Agathos Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses Dwight E. Lynn PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES Chapter 10 Small Scale Protein Production With the Baculovirus Expression Vector System Steve Harwood Chapter 11 Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus Expression Vector System Cynthia B. Elias, Barbara Jardin, and Amine Kamen Chapter 12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors Sue H. Kadwell and Philip I. Hardwicke Chapter 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae Yi Liu, Nathan DeCarolis, and Nikolai van Beek Chapter 14 Virus-Like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated Virus Based Products Marc G. Aucoin, Danielle Jacob, Parminder S. Chahal, Jamal Meghrous, Alice Bernier, and Amine A. Kamen PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression Robert L. Harrison and Donald L Jarvis Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression Lucy Cherbas and Peter Cherbas Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing Robert L. Harrison and Donald L Jarvis PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides Bryony C. Bonning and Tyasning Nusawardani Chapter 19 Baculovirus Insecticide Production in Insect Larvae Nikolai van Beek and David C. Davis Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses Huarong Li and Bryony C. Bonning PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy Hyung Joon Cha and William E. Bentley Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production Yu-Chen Hu and William E. Bentley Chapter 23 Methods For Gene Silencing with RNAi John C. March and William E. Bentley Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis Nor Chejanovsky Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells Christian Hofmann