Bacteriophage Therapy: From Lab to Clinical Practice

ISBN-13: 9781493973941 / Angielski / Twarda / 2017 / 303 str.

Joana Azeredo; Sanna Sillankorva

Opis

This volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacteriophage Therapy: From Lab to Clinical Practice aims to ensure successful results in the further study of this vital field.

Spis treści

Part I: Isolation of Bacteriophages

1. Isolation of Bacteriophages for Fastidious Bacteria

Shigenobu Matsuzaki, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Takako Ujihara, and Masanori Daibata

 

2. Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides

Cristina García-Aljaro, Maite Muniesa, and Juan Jofre

 

3. Isolation of Bacteriophages for Clinically Relevant Bacteria

Sanna Sillankorva

 

Part II: Characterization of Bacteriophages

4. In Vitro Activity against Planktonic and Biofilm Populations Assessed by Flow Cytometry

Luís Melo, Diana Priscila Pires, and Joana Azeredo

 

5. Observation of Bacteriophage Ultrastructure by Cryo-electron Microscopy  

Ana Cuervo and José L. Carrascosa

 

6. Bacteriophage Taxonomy: an Evolving Discipline

Igor Tolstoy, Andrew M. Kropinski, and J. Rodney Brister

 

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Part III: Bacteriophage Selection and Cocktail Formulation

7. Determination of the Bacteriophage Host Range – Culture Based Approach

Andrey V. Letarov, and Eugene E. Kulikov

 

8. Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage

Libera Latino and Christine Pourcel

9. Guidelines to Compose an Ideal Bacteriophage Cocktail

Maia Merabishvili, Jean-Paul Pirnay, and Daniel De Vos

 

Part IV: Biodistribution, Host Interaction and Clinical Application

10. Interaction of Bacteriophages with Mammalian Cells

Zuzanna Kaźmierczak and Krystyna Dąbrowska

 

11. In Vivo Bacteriophage Biodistribution

Nicolas Dufour, Raphaëlle Delattre, and Laurent Debarbieux

 

12. Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-specific Antibodies

Krystyna Dąbrowska

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13. Bacteriophage Treatment of Infected Diabetic Foot Ulcers

Vera V. Morozova, Yulia N. Kozlova, Denis A. Ganichev, and Nina V. Tikunova

 

14. Compassionate Use of Bacteriophage Therapy for Foot Ulcer Treatment as an Effective Step for Moving Toward Clinical Trials

Randolph Fisha, Elizabeth Kutter, Gordon Wheat, Bob Blasdel, Mzia Kutateladze, and Sarah Kuhl

 

Part V: Production, Purification and Bacteriophage Storage

15. Bacteriophage Production in Bioreactors

Maryam Agboluaje and Dominic Sauvage

 

16. Computational Modelling of Bacteriophage Production for Process Optimization

Konrad Krysiak-Baltyn and Sally L. Gras

 

17. Methods for Bacteriophage Preservation

Małgorzata B. Łobocka, Aleksandra Głowacka, and Piotr Golec

 

Part VI: Safety and Regulation

18. Bacteriophage Production in Compliance with Regulatory

Requirements

Jean-Paul Pirnay, Maia Merabishvili, Hilde Van Raemdonck, Daniel De Vos, and Gilbert Verbeken

 

19. Guidelines for Bacteriophage Product Certification

Alan Fauconnier

 

Part VII: New Phage Therapy Approaches

20. Nano/micro Formulations for Bacteriophage Delivery

Pilar Cortés, Mary Cano-Sarabia, Joan Colom, Jennifer Otero, Daniel Maspoch, and Montserrat Llagostera

 

21. Synthetic Biology to Engineer Bacteriophage Genomes

Ana Rita Costa, Catarina Milho, Joana Azeredo, and Diana Priscila Pires