Bacterial and Archaeal Motility

ISBN-13: 9781071630594 / Angielski / Twarda / 2023 / 402 str.

Tohru Minamino; Makoto Miyata; Keiichi Namba

Opis

This detailed volume presents cutting-edge research protocols to study the structure and dynamics of bacterial and archaeal motility systems using bacterial genetics, molecular biology, biochemistry, biophysics, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a section on bacterial flagellar protein export and assembly, the book continues with chapters covering flagella-driven motility of bacteria, archaella-driven motility of archaea, type IV-driven twitching motility of bacteria, as well as adhesion-based gliding motility of bacteria and other unique motility systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and thorough, Bacterial and Archaeal Motility is the ideal reference for researchers working in this vital area of microbiology.

Spis treści

Part I: Bacterial Flagellar Protein Export and Assembly

 

1. Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System

            Miki Kinoshita, Keiichi Namba, and Tohru Minamino

 

2. In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles

            Katsumi Imada and Hiroyuki Terashima

 

3. Molecular Simulation to Investigate Open‒Close Motion of a Flagellar Export Apparatus Protein FlhA_C

            Akio Kitao

 

4. Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy

            Xiang-Yu Zhuang, Chao-Kai Tseng, and Chien-Jung Lo

 

5. Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament

            Tomoko Yamaguchi, Tomoko Miyata, Fumiaki Makino, and Keiichi Namba

 

Part II: Flagella-Driven Motility of Bacteria

 

6. Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy

            Tatsuro Nishikino and Yohei Miyanoiri

 

7. Site-Directed Crosslinking between Bacterial Flagellar Motor Proteins In Vivo

            Hiroyuki Terashima, Michio Homma, and Seiji Kojima

 

8. Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor

            Yusuke V. Morimoto and Tohru Minamino

 

9. Purification of the Na⁺-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy

            Seiji Kojima, Michio Homma, and Hideki Kandori

 

10. Purification of Na⁺-Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy

            Naoya Terahara and Noriyuki Kodera

 

11. High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook

            Shuichi Nakamura and Tohru Minamino

 

12. Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility

            Yukako Eguchi and Azuma Taoka

 

13. Swarming Motility Assays in Salmonella

            Jonathan D. Partridge and Rasika M. Harshey

 

14. Analysis of Adhesion and Surface Motility of a Spirochete Bacterium

            Shuichi Nakamura, Jun Xu, and Nobuo Koizumi

 

15. Force Measurement of Bacterial Swimming Using Optical Tweezers

            Keigo Abe, Kyosuke Takabe, and Shuichi Nakamura

 

Part III: Archaella-Driven Motility of Archaea

 

16. Archaella Isolation

            Shamphavi Sivabalasarma, João N. de Sousa Machado, Sonja-Verena Albers, and Ken F. Jarrell

 

17. Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques

            Yoshiaki Kinosita

 

Part IV: Type IV-Driven Twitching Motility of Bacteria

 

18. In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography

            Longsheng Lai, Yee-Wai Cheung, Matthew Martinez, Kathryn Kixmoeller, Leon Palao III, Stefan Steimle, Meng-Chiao Ho, Ben E Black, Erh-Min Lai, and Yi-Wei Chang

 

19. Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope

            Bingxin Wang, Xiaolong Shao, and Guoliang Qian

 

20. Live Cell Imaging of the Twitching Motility of Cyanobacteria by High-Resolution Microscopy

            Daisuke Nakane

 

Part V: Adhesion-Based Gliding Motility of Bacteria

 

21. Isolation and Visualization of Gliding Motility Machinery in Bacteroidota

            Satoshi Shibata and Daisuke Nakane

 

22. Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniae Under High-Resolution Microscopy

            Daisuke Nakane and Satoshi Shibata

 

23. Social Motility Assays of Flavobacterium johnsoniae

            Chikara Sato and Keiko Sato

 

24. Visualization of Peptidoglycan Structures of Escherichia coli by Quick-Freeze Deep-Etch Electron Microscopy

            Yuhei O. Tahara and Makoto Miyata

 

Part VI: Unique Motility System in Bacteria

 

25. Purification and Structural Analysis of the Gliding Motility Machinery in Mycoplasma mobile

            Takuma Toyonaga and Makoto Miyata

 

26. Motility Assays of Mycoplasma mobile Under Light Microscopy

            Taishi Kasai and Makoto Miyata

 

27. Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile

            Yoshiaki Kinosita, Mitsuhiro Sugawa, Makoto Miyata, and Takayuki Nishizaka

 

28. Direct Measurement of Kinetic Force Generated by Mycoplasma

            Masaki Mizutani and Makoto Miyata

 

29. Genetic Manipulation of Mycoplasma pneumonia

            Tsuyoshi Kenri

 

30. Purification and ATPase Activity Measurement of Spiroplasma MreB

            Daichi Takahashi, Ikuko Fujiwara, and Makoto Miyata

 

31. Swimming Motility Assays of Spiroplasma

            Daisuke Nakane

 

32. Motility Assays of Chloroflexus

            Shin Haruta, Hinata Kakuhama, Shun-ichi Fukushima, and Sho Morohoshi